Sequences from confirmation amplicon


Confirmation sequences for line 419F10 and hit At4g18610 are shown.
Line ID 419F10
Locus At4g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10251271
Primers used for PCR IS59 - 8474, show primer details
   
Sequence Name 39-K023620-022-419-F10C-8409
GenBank Accession LN503213 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K023620-022-419-F10C-8409
AGATTTCCCGGAACATGAAGCCATTTACAATTGAATATATCCATGAATCCCACGAGCCTT
GGCTTGAGATTCTCTCACTTCCCTCAAGTGAACCCGGATCGACCCGTTTGCAAACGGGTT
AGTATCAGGTGACCCGCCACCGTGTTCCTCGTAAGCAGCTCTTAGCCGTCCGATCAAAGC
ATCTAGGCTTCCCCAAGCTTGTTTGAGAGGACACGTGCACGGACCTGGTGGATCCGGTTG
TCCGAAGAAGACACAAGCTTGATGATGTACTTTGGTCTTACCAAACTGATCTAAGTACCT
TAGGAAACTTAGCACGTGCGTGTAGTCGAATTGAGACATCATCAACGGTGGATTTTGTGA
TTTTAGGTATTGGACGAACGTGTTCCAGTCGCGGCGTTTCTGCGATTCATAGCGGCTGAG
TGGTTGCTGCGGTTGAGGAGGAAGTGGTTGCTTGTGGTGGTTGGAGGAAGAAGACGGATG
ATCCGGTGGATCTTTCGTCGGTGTGTGACGATCCGAAGACATTATTAGGGGAATTCTGAT
TTTTTGTTTGTTTGTTTTGATATTATATATTTGGTTTATGAGCAAATTGAAGGTTTAAAA
GCAACAATAAAGTAAGGGAAAGAAAGAGAGATACTGAGAAATAATGGAATGGGA
   
Sequence Name 40-K023620-022-419-F10C-IS59
GenBank Accession LN503570 [GenBank]
Sequencing primer gene specific primer, IS59: 5'-TTTTCGTGAATAATTCTCTCTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K023620-022-419-F10C-IS59
CATTATTTCTCAGTATCTCTCTTTCTTTCCCTTACTTTATTGTTGCTTTTAAACCTTCAA
TTTGCTCATAAACCAAATATATAATATCAAAACAAACAAACAAAAAATCAGAATTCCCCT
AATAATGTCTTCGGATCGTCACACACCGACGAAAGATCCACCGGATCATCCGTCTTCTTC
CTCCAACCACCACAAGCAACCACTTCCTCCTCAACCGCAGCAACCACTCAGCCGCTATGA
ATCGCAGAAACGCCGCGACTGGAACACGTTCGTCCAATACCTAAAATCACAAAATCCACC
GTTGATGATGTCTCAATTCGACTACACGCACGTGCTAAGTTTCCTAAGGTACTTAGATCA
GTTTGGTAAGACCAAAGTACATCATCAAGCTTGTGTCTTCTTCGGACAACCGGATCCACC
AGGTCCGTGCACGTGTCCTCTCAAACAAGCTTGGGGAAGCCTAGATGCTTTGATCGGACG
GCTAAGAGCTGCTTACGAGGAACACGGTGGCGGGTCACCTGATACTAACCCGTTTGCAAA
CGGGTCGATCCGGGTTCACTTGAGGGAAGTGAGAGAATCTCAAGCCAAGGCTCGTGGGAT
TCATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAN
AAATGTAGATGTCCGCAGCG

Last Updated on 06.12.2018 16:11