Sequences from confirmation amplicon


Confirmation sequences for line 419F11 and hit At2g29580 are shown.
Line ID 419F11
Locus At2g29580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12652020
Primers used for PCR PB30 - 8474, show primer details
   
Sequence Name 51-K102504-0022-419-F11C-8409
GenBank Accession LN507460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K102504-0022-419-F11C-8409
TTACAATTGAATATATCCTGAGAATACTGAGAGATCACGAGGCTGATGGATGGGAACGTT
CTGATTTCCCAATCATCTGTGAGTCTTGTCTCGGTGACAATCCTTACGTGAGAATGGTAT
ATTCTTCGCTTCCTATGTTTCTCCTCAAACAAATTCGATTTCCTTGCCTTTAGCTAGATT
TTTTTTTAACATTCATGTGTCTTTATTAAGATCTCCTTTCATTTGTCCATTTTCGAGCTT
GTTTAATGCTTTTATGTTTCTTAGTGTTGAAGCTGCAATTTTAGTGATTTGAGATTTTAG
GGTTTAAGCGATTTAATATACAATTCATAACCTTTGGTAAAAATCAGAATTCATGGAGTT
GTAGTTCAAAATGATATCTTTGGTTTGTTCTGATCAAATTTGCTTAGAGGGAATGTAATT
CATTGAATACTGTCTCATGTTATGTCTGGTATGNATGTGC
   
Sequence Name 51-K102505-0022-419-F11C-PB30
GenBank Accession LN507461 [GenBank]
Sequencing primer gene specific primer, PB30: 5'-TGCACATCACTACCAGACATAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K102505-0022-419-F11C-PB30
TTCCCTCTAAGCAATTTGATCAGAACAACCAAAGATATCATTTTGAACTACAACTCCATG
AATTCTGATTTTTACCAAAGGTTATGAATTGTATATTAAATCGCTTAAACCCTAAAATCT
CAAATCACTAAAATTGCAGCTTCAACACTAAGAAACATAAAAGCATTAAACAAGCTCGAA
AATGGACAAATGAAAGGAGATCTTAATAAAGACACATGAATGTTAAAAAAAAATCTAGCT
AAAGGCAAGGAAATCGAATTTGTTTGAGGAGAAACATAGGAAGCGAAGAATATACCATTC
TCACGTAAGGATTGTCACCGAGACAAGACTCACAGATGATTGGGAAATCAGAACGTTCCC
ATCCATCAGCCTCGTGATCTCTCAGTATTCTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11