Sequences from confirmation amplicon


Confirmation sequences for line 420A10 and hit At1g12270 are shown.
Line ID 420A10
Locus At1g12270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4173909
Primers used for PCR SP26 - 8474, show primer details
   
Sequence Name 50-K103548-0022-420-A10C-SP26
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, SP26: 5'-ATATTTCGATCCTAAGCTAGGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K103548-0022-420-A10C-SP26
GCTTTACATTAGTCTCTTTTTTTTTTTTTGGGGGGGTGTGTGTGTGTGGGGGGGAAAAAA
AAACCTTTAAAACCCCCGAAAAAAAAAAAGAATTTCCCTTTTTCCCGGCCGCTGTGGGGG
TTTTTTAAAAACGGGAAAATTTTTCTCCAGGGAAAAAAATTCCCCGGGGCCCAAAAAATT
TCCCCCCAAACCATCAAAAGGAACCCAAAAGACCCAAAAAGAGGGGCCCCTCCCCCATTT
TTTTTTTTTCTAAGGGCCTCCAAAAATTTTTTTCTTTCCCAAGGGTTAAACCACCCCTTT
TTTTTTTCCCCCCTTTTTAGGGAAAAAACCACCCACCCTCCTTTTACCAAAAACCTGGGG
CCACCCCCGAAAGAAAAAAGGGACCAAAAAATTTTTTTGGCCCCCCCCCCCCCCTTCTCA
AAGGAAAACAAAAAAAAACCGCCCCCCATTTTCCCCCCAAAAAAAAAAAACATGGGGGGG
AAAACCCCCCACCCACCCGGAAATTTTCCATTTGAAAGGGCCTCCGGGCCGGGAAATCCC
CCGGGGACCCCCAAGGATAAGGGGGGCCAATGGGAAAAAAAAAAAAAATTATTCCCCTTT
TTTTTTCCTTTCCAAAAGGGTAATTCCCCCCCCGTTTTAAAAAA
   
Sequence Name 34-K103549-0022-420-A10C-8409
GenBank Accession LN501384 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K103549-0022-420-A10C-8409
TTACAATTGAATATATCCTGATTGCTTGGTATGTTTCCATCGCATTGTCGTACTCTTTGA
GGAAGAATTGGACTGCAGCTTTTCTGCTGTATCCTTTAGAGAAGGTTGGATCGAGCTCAA
TACACTTTTCTGCATCCTTTAGTCCTTCAGGCATTGCACCTAGCTTAGTGTAACTAGCAG
CTCGGTTGCTATATGCCTAACATAGGTGCAAAAAAAGTACGAGTTGCTCAAACAGTCGGA
ATAGAAAATCATTGTAGAAGACCTTAAGAACATATAAATATGATGAAGTGCCCTTACTTT
ATGGTCATTTGGGTTCCTTTTGATTGCTTCGGTGTAATGTTTTATGGCCTCAGGATACTT
CTGCTCCTTGAAGAAATCGTTACCTGTATTAAATACACCACAACAGCAGCAGTAAAAACG
CTAATTCATTCTTGTTTTCAGGGTTCTATAAGCTTTCTTTTCCCACACACACACACACAC
ACACACACAAAAAAAAAAAAAAAAATTTTTTGCAAAGCCACCCCTTTTTTCCCCCCTTTC
CCCCCCCGGGTAAAAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11