Sequences from confirmation amplicon


Confirmation sequences for line 420C04 and hit At4g30780 are shown.
Line ID 420C04
Locus At4g30780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14991914
Primers used for PCR 10yd - 8474, show primer details
   
Sequence Name 05-K120060-0022-420-C04C-10yd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10yd: 5'-TGAATTTAGTTATGGAGTCCTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K120060-0022-420-C04C-10yd
AGCTAGTATACCCGCTTGATGAGCCGAGTCCTCTTGGATTGAGTCTTAAGAAGAGTCCAT
CTTTGTTGGAGTTGATTCAGATGAAGATAACGCATTGTGGTGATCCTAAAGCAGCTGAAA
CGTTGAAAGCTGGTGCACTTGGCTCTGGTCTTAAGAGAGAAAGTAAAACTATTGCTGCTG
CTGCATCAGTTGGACCGACTTTGGCGCCAGGAAGTATTGAGAAGCTGAAAGCTTCTAACT
TTCCTGCTTCGCTTTTGAAGATCGGGCAGTGGGAGGTGAGTCCTGCGAAATGTTTCGTTT
TGATATGTTTTCATGGTGGTTTCAAAATCTGACTTGACTTGGTTTCTTTTTTTGTGCAGT
ATAAATCGAGGTATGAAGGTGATTTGGTGGCAAAGTGTTACTTTGCTAAACACAAACTTG
TTTGGGAAGTGTTGGAACAAGGTCTTAAGAGTAAAATCGAGATCCAGTGGTCAGATATTA
TGGCTTTGAAGGCAAACTGTCCTGAAGACGGACCTGGAACTTTGACTCTCGTGGTATAAC
ATTCACTCATGTTCTTTTCTTTTGCTCAACATATCACTCTTGTTCAACTTTAATGATTTG
ACTCTTTATGTTTCAGCTTGCTAGACAGCCCTTATTTTTCCGGGAAACAAACCCACAGCC
TAGAAAGCATACCTTGTGGCAGGCGACATCAGATTTCACTGATGGCCAAGCCAGCATGAA
CAGGTACAGGCATTTCATGTAAATTTTTTCTGAATAAATTTGGTGATGTCCTAGCTTCAG
CTAACGATAGTCTCCCTGTGATCTGTTAGGCAGCATTTTCTGCAATGTGCTCAAGGGATA
ATGAACAAACATTTTGAAAAGCTCGTTCAATGTGATCATCGTCTGTTCCATCT
   
Sequence Name 01-K120059-0022-420-C04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K120059-0022-420-C04C-8409
TCATTGAAGTCTATTTCATTTACATTAAATTCTGAGTCCCAGAGATCGAAGGACCTGTAC
TAAGATTCAAATTCCCAAAAGGATGGCCTTTTGATTCACTTGGATCCTCAAAAATAGATT
GCCGTGCATCAAAGTAGGGAGAGTCAATAGCTATCTCCGGCTGTCGGCTTAGATGGAACA
GACGATGATCACATTGAACGAGCTTTTCAAAATGTTTGTTCATTATCCCTTGAGCACATT
GCAGAAAATGCTGCCTAACAGATCACAGGGAGACTATCGTTAGCTGAAGCTAGGACATCA
CCAAATTTATTCAGAAAAAATTTACATGAAATGCCTGTACCTGTTCATGCTGGCTTGGCC
ATCAGTGAAATCTGATGTCGCCTGCCACAAGGTATGCTTTCTAGGCTGTGGGTTTGTTTC
CCGGAAAAATAAGGGCTGTCTAGCAAGCTGAAACATAAAGAGTCAAATCATTAAAGTTGA
ACAAGAGTGATATGTTGAGCAAAAGAAAAGAACATGAGTGAATGTTATACCACGAGAGTC
AAAGTTCCAGGTCCGTCTTCAGGACAGTTTGCCTTCAAAGCCATAATATCTGACCACTGG
ATCTCGATTTTACTCTTAAGACCTTGTTCCAACACTTCCCAAACAAGTTTGTGTTTAGCA
AAGTAACACTTTGCCACCAAATCACCTTCATACCTCGATTTATACTGCACAAAAAAAGAA
ACCAAGTCAAGTCAGATTTTGAAACCACCATGAAAACATATCANAACGAAACATTTCGCA
GGACTCACCTCCCACTGCCCGATCTTCAAAAGCGAAGCAGGAAAGTTAGAAGCTTTCAGC
TTCTCAATACTTCCTGGCGCCNAAGTCGGTCCAACTGATGCAGCAGCAGCAATAGTTTT

Last Updated on 06.12.2018 16:11