Sequences from confirmation amplicon


Confirmation sequences for line 420C04 and hit At5g47635 are shown.
Line ID 420C04
Locus At5g47635 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19307632
Primers used for PCR 63rq - 8474, show primer details
   
Sequence Name 58-K116249-0022-420-C04C-63rq
GenBank Accession LN509801 [GenBank]
Sequencing primer gene specific primer, 63rq: 5'-ACTTTTGTACCAAAAACATGGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K116249-0022-420-C04C-63rq
CTTTGGGACATGAAACACAATGTAGAGAGACTCTAACTCGGTTCTGTGACTGGAGCGCCA
GCTAAGCAAACGAGGACCATAACAAAAAAAATGCTTTTCACATGAAGGAACTTGAAGAAT
CAGCTGTTTGTGTTCAAATCTTTTGGTTGAAATGTTGTCTCAACGAGCTTGCTATGATCT
CGAACTGTGGCCCTGTAACCTGATTTTCCCTGAGGTGTAGACACGGAAACCATTGGTAGA
GGAAACAAGTTTGATAGGTTTGTGTATATTGGTAGAGGTGTTATAGCATCTATAGGGCCT
AGGCACATATACTGGCTTGATGAAACAAGTGTTTTCAAGGTGTGGAATCGCATGGAGTTG
GGAGGGAAGATCGATTTCAAACTCTCCCAATTCATCAGTAACAGCCTTAATCCATTTGGA
CCTCCTTTTGGATCCAGTGTGGCACTTGATGGCAACGGTGGCACCTGCGAGGATCATGTT
AGTATATATTCGGACGGAAGACAAGAAACATGAAGAAAAAAGGTAGAGAAACAAATACCT
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 34-K116248-0022-420-C04C-8409
GenBank Accession LN501554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K116248-0022-420-C04C-8409
CCTTTTTTCTTCTGTTTCTTGTCTTCCGTCCGAATATATACTAACATGATCCTCGCAGGT
GCCACCGTTGCCATCAAGTGCCACACTGGATCCAAAAGGAGGTCCAAATGGATTAAGGCT
GTTACTGATGAATTGGGAGAGTTTGAAATCGATCTTCCCTCCCAACTCCATGCGATTCCA
CACCTTGAAAACACTTGTTTCATCAAGCCAGTATATGTGCCTAGGCCCTATAGATGCTAT
AACACCTCTACCAATATACACAAACCTATCAAACTTGTTTCCTCTACCAATGGTTTCCGT
GTCTACACCTCAGGGAAAATCAGGTTACAGGGCCACAGTTCGAGATCATAGCAAGCTCGT
TGAGACAACATTTCAACCAAAAGATTTGAACACAAACAGCTGATTCTTCAAGTTCCTTCA
TGTGAAAAGCATTTTTTTTGTTATGGTCCTCGTTTGCTTAGCTGGCGCTCCAGTCACAGA
ACCGAGTTAGAGTCTCTCTACATTGTGTTTCATGTTCCCAAAGTGTAAGAACAAACAACT
GTAAGCATCATGTATATAAAGGCTCCCATGTTTTTGGAACAAAAGTA

Last Updated on 06.12.2018 16:11