Sequences from confirmation amplicon


Confirmation sequences for line 420G03 and hit At4g02390 are shown.
Line ID 420G03
Locus At4g02390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1053506
Primers used for PCR CN02 - 8474, show primer details
   
Sequence Name 11-K023835-022-420-G03C-8409
GenBank Accession LN490933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K023835-022-420-G03C-8409
CCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATGAATGAACTTCTGTATTCAG
ATTATAACGCGGATAATCTACCCCCGGGAAAGCTAAGGTATGAAGATAATCTCATTCATA
TTTCACACAAGTTCCTTATCTCAAACCGCAATGAATTATGATTTACTATTTTTGTACTGT
ACCTTGATTTGTTATTTGATGGTTGCAGCACAAAAGGTGTGGGGAAAACAGCACCAAACC
CATCAGAGGCTCAAACACTAGAAGACGGTGTTGTTGTTCCACTTGGCAAACCAGTGGAAC
GTTCATGCTCCAAGGTTTTTGTTTTTGTCTTGCTTCAACACACATTCACAAAATCTAACT
CTCTCAAATTTCATAAACTGACTTTATATAACTTTTGCAGGGGATGTTGTTGTACAACGA
ATATATAGTCTACAATGTGGAACAAATCAAGATGCGTTATGTGATCCAAGTCAAATTCAA
CTACAAGCACTAAAACTTATGTATATTAGCTTTTGAACATCAACTAATTATCCAAAAATC
AGCGTTTTATTGTATTTCTTTCAAACTCCTTCATCTCTGATTTTGCACGGTTCACTCGTA
TATTGGTACGCTAAACCGCAAACCATACCCAATT
   
Sequence Name 15-K023835-022-420-G03C-CN02
GenBank Accession LN492790 [GenBank]
Sequencing primer gene specific primer, CN02: 5'-CTAAACCGGATAAAATTGGGTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K023835-022-420-G03C-CN02
GGTTTAGCGTCCAATATACGAGTGAACCGTGCAAAATCAGAGATGAAGGAGTTTGAAAGA
AATACAATAAAACGCTGATTTTTGGATAATTAGTTGATGTTCAAAAGCTAATATACATAA
GTTTTAGTGCTTGTAGTTGAATTTGACTTGGATCACATAACGCATCTTGATTTGTTCCAC
ATTGTAGACTATATATTCGTTGTACAACAACATCCCCTGCAAAAGTTATATAAAGTCAGT
TTATGAAATTTGAGAGAGTTAGATTTTGTGAATGTGTGTTGAAGCAAGACAAAAACAAAA
ACCTTGGAGCATGAACGTTCCACTGGTTTGCCAAGTGGAACAACAACACCGTCTTCTAGT
GTTTGAGCCTCTGATGGGTTTGGTGCTGTTTTCCCCACACCTTTTGTGCTGCAACCATCA
AATAACAAATCAAGGTACAGTACAAAAATAGTAAATCATAATTCATTGCGGTTTGAGATA
AGGAACTTGTGTGAAATATGAATGAGATTATCTTCATACCTTAGCTTTCCCGGGGGTAGA
TTATCCGCGTTATAATCTGAATACAGAAGTTCATTCATTTCAGGATATATTCAATTGTAA
A

Last Updated on 06.12.2018 16:11