Sequences from confirmation amplicon


Confirmation sequences for line 421G12 and hit At5g13220 are shown.
Line ID 421G12
Locus At5g13220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4220016
Primers used for PCR HF16 - 8474, show primer details
   
Sequence Name 50-K032418-022-421-G12C-8409
GenBank Accession LN507067 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K032418-022-421-G12C-8409
ATTTACAATTAAAGGGATTTTTACCCCCAATAATTAAAGAAAGGTTTTTATTTTTCTCTC
TCTCGACCTTTTTTTTACTATAAGTTATTTAAGATAGTAATTATGGGTCCTGCCTCTTTT
ACTCTCACATACAACTTAAGATTCAACTAGTTTTGTTCAACAACGCACATGCTTATACGT
AGATAGATAATGGAGATCAGTAGTAATATCGGTATACGTAGGTTACTATTGTAATGGAAC
TTTTAAAAAGCGCGTTGACTTTGAGTCTTTGACTCTAGTTCTGTTTGCTACACCGACAAG
TTATATTTTTCAAAATGATGAGAAAACNAAGGAGAA
   
Sequence Name 50-K032418-022-421-G12C-HF16
GenBank Accession LN507068 [GenBank]
Sequencing primer gene specific primer, HF16: 5'-TTTCTCCTCGTTTTCTCATCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K032418-022-421-G12C-HF16
CAACGAACTGAGTCAAAGACTCAAAGTCAACGCGCTTTTTAAAAGTTCCATTACAATAGT
AACCTACGTATACCGATATTACTACTGATCTCCATTATCTATCTACGTATAAGCATGTGC
GTTGTTGAACAAAACTAGTTGAATCTTAAGTTGTATGTGAGAGTAAAAGAGGCAGGACCC
ATAATTACTATCTTAAATAACTTATAGTAAAAAAAAGGTCGAGAGAGAGAAAAATAAAAA
CCTTTCTTTAATTATTTGGGGGTAAAAATCCCTTTAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11