Sequences from confirmation amplicon


Confirmation sequences for line 422A01 and hit At1g33990 are shown.
Line ID 422A01
Locus At1g33990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12356591
Primers used for PCR 27gp - 8474, show primer details
   
Sequence Name 29-K112248-0022-422-A01C-27gp
GenBank Accession LN499354 [GenBank]
Sequencing primer gene specific primer, 27gp: 5'-TTTCTAATGGCAAGCAGGTTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112248-0022-422-A01C-27gp
CTCCCTTATAAGTTTCATCTTGAAAGTTCAAAGGTTTCATTTTTATATGATTTAATGATC
GTTTCGACTGAGTCTTTGCCTTTTTGCCGGTTTTAATGATTGTTTGGGAACTTTTTATAT
ATTTTGGTTACTATAAAAGTGGAGGAAGAGAAGATTCTGTGTTAAGATTAGAAAAGAGAT
GTGAAAAAGTGTTGTAAGATTTCTGACTTTTTTGTTGGTAATGTTAACTTTATCATGAAA
TGAAAAGTTCTAAACTCTGATACTGATACTGACACTGGGTTGGGACCAGATGAGCTATAA
AACTATGGAGTGTCTGTCTTTAGCCACACCCCATAACCTTACAACAGAAGTACTGATAAA
CTTTTAATTGTCTTTCAATTGCATTTTGTCTCTTCTACAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 13-K112247-0022-422-A01C-8409
GenBank Accession LN492103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112247-0022-422-A01C-8409
CCTGTAGAAGAGACAAAATGCAATTGAAAGACAATTAAAAGTTTATCAGTACTTCTGTTG
TAAGGTTATGGGGTGTGGCTAAAGACAGACACTCCATAGTTTTATAGCTCATCTGGTCCC
AACCCAGTGTCAGTATCAGTATCAGAGTTTAGAACTTTTCATTTCATGATAAAGTTAACA
TTACCAACAAAAAAGTCAGAAATCTTACAACACTTTTTCACATCTCTTTTCTAATCTTAA
CACAGAATCTTCTCTTCCTCCACTTTTATAGTAACCAAAATATATAAAAAGTTCCCAAAC
AATCATTAAAACCGGCAAAAAGGCAAAGACTCAGTCGAAACGATCATTAAATCATATAAA
AATGAAACCTTTTGAACTTTTCAAGATTGAAACTTTTATAAGTGTGGAGAAGATTGAGAA
TCAGTAGTGACTTAACCTGC

Last Updated on 06.12.2018 16:11