Sequences from confirmation amplicon


Confirmation sequences for line 423A09 and hit At4g26830 are shown.
Line ID 423A09
Locus At4g26830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13494897
Primers used for PCR 33qv - 8474, show primer details
   
Sequence Name 75-K115779-0022-423-A09C-33qv
GenBank Accession LN513291 [GenBank]
Sequencing primer gene specific primer, 33qv: 5'-CAGCATTCATGCAAGATTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K115779-0022-423-A09C-33qv
ATTTTAATTAAATAATAAAATAGTTCAACAAAGTTAATATATTTTATACACAATATAAAA
TATATATATATTCCCGATTAGCAAACAATGTACTTAATAAATCAGGCAAAAAAAAAAACC
CAAAAAATTGCTGCCAACTATTCCCCCCCAATGTCCGGGGGAAAACTCCCATTTTCCATA
TCCGGCAATCAACAAAAAAATAATTTTGTTAAAATTATATAAAAATATTAAGGGTCCCCA
TATATTTCGATCTTACAAAAACGTGAACTTCTAAAAAGGCCAAAATGACCCAATATACCT
AAATTTTCACAACCCCCAATTATCATTTGAAAATGGCTCCATGTCCGGAAAATCTCCTGG
GATCACCAATGAGTATAATGGTCAATATGGAAAAAAAAAAAAAGTAATTCCCATTTTTTT
TTCATTTCAAAAATGTAAATTTCCCAAACTTTTTA
   
Sequence Name 27-K115778-0022-423-A09C-8409
GenBank Accession LN498535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115778-0022-423-A09C-8409
TCGGACTGAGCATTTACAATTGATAATTGGCGGTTGTGAAAGTTGAGCTATATTGGGTCA
TTTTGGCCTTTTAAGATGTTCACGTTTCTGTAAGATCGATATATATGGTGACCTTTAATA
TTTTTATATAATTCTAACAAAATTATTTTTTCGTTGATTGCAGGATATGGAAAATGCGAG
TTTCCCACCGGACATTGAGGCGGAATAGTTGGCAGCAATTTTCTGTGTTTTTTTTTTTGC
CGGATTTATTAAGTACTTTGTTGGCAAATCGGGAATATATATATTTTTTATATGGGGTAT
AAAATATATAAACTTTGTTGAACTATTTTATATATTTTAATTTAAAATTATATCACCCCT
AAATTATTCTCAAAATCTGGCAGGAAGGCGG

Last Updated on 06.12.2018 16:11