Sequences from confirmation amplicon


Confirmation sequences for line 423B01 and hit At5g13550 are shown.
Line ID 423B01
Locus At5g13550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4359362
Primers used for PCR RK91 - 8474, show primer details
   
Sequence Name 25-K022407-022-423-B01C-8409
GenBank Accession LN497257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K022407-022-423-B01C-8409
TAGATTTCCCGGACATGAAGCCATTGGAGAATCCGTTAAAGAGCTTTGTATCATTTATTC
CATCAATGCTCATAAGCTTTACTCTAGTGTCTGGTCAATTTTGTTTTTTTTCAAACTAAA
CTTTACAATTATCATTACATTATCTAATCATACGTCAAAAATAATTTAGTTTATAGTAAT
AGATCCAGAATTACGATAACGTAACTACATTTAAATTTTGCTAATCACATAACATTTTTA
AAATTAGTTGTTAATTAGTAATTAGATAAATTGTTGTCACTTGTAAGTTTCGTCACGTAT
CTCTAATTAATAGAATAGAATTCATGAGGGGTCAATCCAATACAAGTGGCTTGCGTGCTA
GTTTTGATGATTTGTTTACTATTCTTTATTTTAATAGGTACCAGAAATTGGGAGGGAATA
AAACATGTGTTGAAAAAAGTGTGAAG
   
Sequence Name 26-K022407-022-423-B01C-RK91
GenBank Accession LN497735 [GenBank]
Sequencing primer gene specific primer, RK91: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K022407-022-423-B01C-RK91
GTTTTATTCCCTCCCAATTTCTGGTACCTATTAAAATAAAGAATAGTAAACAAATCATCA
AAACTAGCACGCAAGCCACTTGTATTGGATTGACCCCTCATGAATTCTATTCTATTAATT
AGAGATACGTGACGAAACTTACAAGTGACAACAATTTATCTAATTACTAATTAACAACTA
ATTTTAAAAATGTTATGTGATTAGCAAAATTTAAATGTAGTTACGTTATCGTAATTCTGG
ATCTATTACTATAAACTAAATTATTTTTGACGTATGATTAGATAATGTAATGATAATTGT
AAAGTTTAGTTTGAAAAAAAACAAAATTGACCAGACACTAGAGTAAAGCTTATGAGCATT
GATGGAATAAATGATACAAAGCTCTTTAACGGATTCTCCAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
Primers used for PCR RK90 - 8474, show primer details
   
Sequence Name 33-K022408-022-423-B01C-8409
GenBank Accession LN500825 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K022408-022-423-B01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTTACAAAGTCATTGATGGAATAAATGACA
CCTCCAATGAAGCTCGTCCTCCTTCGATTCCTTTCGACGATATCTTCTCCGGATGGACGG
CGAAGATCAAGCGCATGAGGCTCGTCGATTGGATTGATACTCTCTTTCCTTGTTTCCGAT
GGATTCGAACTTACCGATGGAGCGAGTATTTTAAGCTCGATCTCATGGCTGGTATCACCG
TCGGTATAATGCTTGTTCCCCAGGTACTTTACTTTCAAGCTTAAGGAAGCTGGAATCATT
GGCTTTAGAATAGGAAACTGGAATTATTGGTTTCAATCGTATTGTCTTGTTGAGCTGCAA
AATTGGTTAAAGATGTGACAAAATGTATTATAGCTTAAGAGATTATCAATTCAAGTTTTT
AGTAAAATTATGTATTGGTCTTCAATCTGGAGTTTTGTTTTAAGTTTATTTTGCTGAATG
ACTTTTGATGGATTTGACCAGGCAATGTCGTATGCGAAACTAGCTGGCCTTCCACCAAT
   
Sequence Name 34-K022408-022-423-B01C-RK90
GenBank Accession LN501249 [GenBank]
Sequencing primer gene specific primer, RK90: 5'-ACATAGACCGTAAATTGGTGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K022408-022-423-B01C-RK90
GCTAGTTTCGCATACGACATTGCCTGGTCAAATCCATCAAAAGTCATTCAGCAAAATAAA
CTTAAAACAAAACTCCAGATTGAAGACCAATACATAATTTTACTAAAAACTTGAATTGAT
AATCTCTTAAGCTATAATACATTTTGTCACATCTTTAACCAATTTTGCAGCTCAACAAGA
CAATACGATTGAAACCAATAATTCCAGTTTCCTATTCTAAAGCCAATGATTCCAGCTTCC
TTAAGCTTGAAAGTAAAGTACCTGGGGAACAAGCATTATACCGACGGTGATACCAGCCAT
GAGATCGAGCTTAAAATACTCGCTCCATCGGTAAGTTCGAATCCATCGGAAACAAGGAAA
GAGAGTATCAATCCAATCGACGAGCCTCATGCGCTTGATCTTCGCCGTCCATCCGGAGAA
GATATCGTCGAAAGGAATCGAAGGAGGACGAGCTTCATTGGAGGTGTCATTTATTCCATC
AATGACTTTGTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
Primers used for PCR 23km - 3144, show primer details
   
Sequence Name 70-K117894-0022-423-B01C-23km
GenBank Accession LN512457 [GenBank]
Sequencing primer gene specific primer, 23km: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K117894-0022-423-B01C-23km
ATTTCTGGTCCTATAAAATAAAGAATAGTAAACAAATCATCAAAACTAGCACGCAATCCA
CTTGTATTGGATTGACCCCTCATGAATTCTATTCTATTAATTAGAGATACGTGACGAAAC
TTACAAGTGACAACAATTTATCTAATTACTAATTAACAACTAATTTTAAAAATGTTATGT
GATTAGCAAAATTTAAATGTAGTTACGTTATCGTAATTCTGGATCTATTACTATAAACTA
AATTATTTTTGACGTATGATTAGATAATGTAATGATAATTGTAAAGTTTAGTTTGAAAAA
AAACAAAATTGACCAGACACTAGAGTAAAGCTTATGAGCATTGATGGAATAAATGATACA
AAGCTCTTTAACGGATTCTCCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCG
ATGCCTTGATTTCGCCATTCCCAGATACCCATTTCATCTTCAGATTGGTCTGAGATTATG
CGAAAATATACACTCATATACATAAATACTGACAGTTTGAGCTACCAATTCAGTGTAGCC
CATTACCTTACATAATTCACTCAAATGCTAGGCAGTCTGTCAACTCGGCGTCAATTTGTC
GGCCACTATACGATAGTTGCGCAAATTTTCAAAGTCCTGGCCTAACATCAC
   
Sequence Name 59-K117893-0022-423-B01C-35St
GenBank Accession LN510097 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117893-0022-423-B01C-35St
CTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTTTTCGTCCATTAGACTATCTATTGTGATGGTGGA
TTTATCACAAATGGGACCCGCCGCCGACAGAGGTGTGATGTTAGGCCAGGACTTTGAAAA
TTTGCGCAACTATCGTATAGTGGCCGACAAATTGACGCCGAGTTGACAGACTGCCTAGCA
TTTGAGTGAATTATGTAAGGTAATGGGCTACACTGAATTGGTAGCTCAAACTGTCAGTAT
TTATGTATATGAGTGTATATTTTCGCATAATCTCAGACCAATCTGAAGATGAAATGGGTA
TCTGGGAATGGCGAAATCAAGGCATCGATCGTGAAGTTTCTCATCTAAGCCCCCATTTGG
ACGTGAATGTAGACACGTCGAAATAAAGATTTCCGAATTAGAATAATTTGTTTATTGCTT
TCGCCTATCAATACGACGGATCGTAATTTGTCGTTTTATCAAAATGTACTTTCATTTTAT
AATAACGCTGCGGACATCTACATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTT
TTTCTCCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGC
CATTGGAGAATCCGTTAAAGAGCTTTGTATCATTTATTCCATCAATGCTCATAAGCTTTA
CTCTAGTGTCTGGTCAATTTTGTTTTTTTTCAAACTAAACTTTTACATTATCATTACATT
ATCTAATCATACGTCAAAAATAGTTTATTTTATAGTAATAGATCCGGAATTACGATAACG
TAACTACATTTAAATTTTGCTAATCACATA

Last Updated on 06.12.2018 16:11