Genotyping and primer details


Genotyping and primer details for 423B01 - At5g13550
Line ID 423B01
Locus At5g13550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4359362 of line 423B01 Chr5:4359362
   
Insertion allele amplicon 3144 - 23km
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 23km: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4359979
   
Insertion allele amplicon 8474 - RK90
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK90: 5'-ACATAGACCGTAAATTGGTGGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4358866
   
Insertion allele amplicon 8474 - RK91
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RK91: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4359979
   
wt amplicon (over InsPos) 23km - RK90
wt amplicon start Chr5:4358866
wt amplicon end Chr5:4359979
wt amplicon size 1114 bp
Locus specific primer RK90: 5'-ACATAGACCGTAAATTGGTGGAAG-3'
2nd locus specific primer 23km: 5'-TTAACGTCCCTCTTCACACTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11