Sequences from confirmation amplicon


Confirmation sequences for line 423F04 and hit At3g60140 are shown.
Line ID 423F04
Locus At3g60140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22220186
Primers used for PCR IW53 - 8474, show primer details
   
Sequence Name 31-K033212-022-423-F04C-IW53
GenBank Accession LN500056 [GenBank]
Sequencing primer gene specific primer, IW53: 5'-TGGGGTACGTATTTACGTCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K033212-022-423-F04C-IW53
TTTAAGCTACTGTAATCATTCTAAGAATTTTAAATTTTGTATTATATATCCTCAGGAAGA
AAGGGGCTTCTTATTTTCACACCCGGAAGGCTTACGAAAAGTTCTTAACTATATTAAAGA
GAGATACAATAACATGCCAGTCTACATCAAAGAAAATGGTCAGAAACTCTTTCCTTTTCT
TTTTTTTGCTAAAGCAGAAAGTTTTTTTTTGTTAACTAGAAATTCAGAAAACTTTTTAGT
ACGTAGTTATTTAACTAAAGATTTTTTTATTTTTTTATTTTTTAACTCTACCACACATTA
ATAATATCCTAGCAGTGAATTGAAACTGAAGTTTGATTTAAATATATTTCCCTAACCAGT
TCAACAAACTGATTTAAAAAAAAAAAAAAAGGGGGGGACTATAAAAAATTTACGTTGATT
ACGGAATTCCGGGAATTAAGGACAACGACAACGGTCCAAAACCAAAAAAAAAATTTGGGA
AGGACCCTTTTGGGATTGAATCCCATAAAACCCTTTTCGAAAATCTCCTGGGATCACCAA
TGAGTATGAGGGTCAATAGGGAAAAAAAAAAAGAGTAATTCCCATTTTTTTTTCAATTCA
AAAATGTAAATGTCCCCACCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAA
TTCCAATCCGTCGTATTTATGGGCAAAAGCAATAAACAAATTATTCTAATTCGAAAATCT
TTATTTCAACGTGTCTACATT
   
Sequence Name 23-K033212-022-423-F04C-8409
GenBank Accession LN496457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033212-022-423-F04C-8409
TTCGAAGTGTCTTAGGTATTCAATCCTAAATGTGTCCTTCACAATTTCTTCTCTTGGTTT
TGTACCGTCGTCGTTGTCATTAATTCCTGTAATTCAGTAATCAACGTAAATTATTAATAG
TCACACCATTTTTTTTTTTTTTAAATCATTTTGTTGAACGGGTTAGGGAAATATATTTAA
ATCAAACTTCAGTTTCAATTCCCGGCTAGGAAATTATTAAGGGGGGGTAAAGTTAAAAAA
TAAAAAAATAAAAAAATCTTTAGTTAAAAAACTCCGTACTAAAAAGTTTTCTGAATTTCT
AGTTAACAAAAAAAAACTTTCTGCTTTACCAAAAAAAAAAAAAGGAAAGAGTTTCGGACC
TTTTTCTTTGAGGTAAACGGGCAGGTTATTGTACCCCCCTTAAAAAAAGTAAAAAACTTT
TCGAAACCCTTCCGGGGGGGAAAAAAAAAACCCCCTTTCTTCCGGGGGATATATAAAACA
AAATTTAAAATACCTTAAAAGGATTCCCTGTACCTTTTGAAATTGATAGTTATGAAAAAA
AAACTTAAATCCTTCCCCCAA

Last Updated on 06.12.2018 16:11