Sequences from confirmation amplicon


Confirmation sequences for line 424A06 and hit At4g05180 are shown.
Line ID 424A06
Locus At4g05180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2673193
Primers used for PCR 19us - 8474, show primer details
   
Sequence Name 47-K117387-0022-424-A06C-19us
GenBank Accession LN506317 [GenBank]
Sequencing primer gene specific primer, 19us: 5'-CTATGTGGCAAAACTGAGAATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117387-0022-424-A06C-19us
AACCATATCCGAGTAATCTAGTAACAACGTGAATCAGATAAGGTATTCTTTGTCACATAA
CCAAACACCAAAAATTATAACTCAACACTTCACTAATCAATTCGTAAAGACTGGCGAACA
GTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGA
GCACGACACGCTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGC
AATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGC
TATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCA
TTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGG
ACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCA
AGTGGATTGATGTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAG
   
Sequence Name 20-K117386-0022-424-A06C-8409
GenBank Accession LN495388 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117386-0022-424-A06C-8409
ATTTCATTGAATATATCACATCAATCCACTTGCTTTGAGACGTGGTTGGAACGTCTTCTT
TTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCAT
CTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTTT
CCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGG
ATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCTGAGACTGTATCTTTGA
TATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTGT
CATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTTACGAATTGATTAGTGA
AGTGTTGAGTTATAATTTTTGGTGTTTGGTTATGTGACAAAGAATACCTTATCTGATTCA
CGTTGTTACTTAGATTACTCGGATATGGTTTTAACTACTAATATGTTGACACGTGGTTTC
TTGATTCTCAGTTTGCCCACATA
Primers used for PCR 52vd - 8474, show primer details
   
Sequence Name 75-K117751-0022-424-A06C-52vd
GenBank Accession LN513298 [GenBank]
Sequencing primer gene specific primer, 52vd: 5'-CAAGAAACCGCTAGAAAACAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K117751-0022-424-A06C-52vd
CATGGTTTGGAAAGCTTTGCGGTGAGATCTTTAAGGCTTTGCTTCTCTTCCTTAGGCTTA
GCGGAGATAACGGTGTTGAGATCGTAACGGAGGTACGATGCTCTTAAACGGAGATCGTTC
TGAACATAGGGCCAAGCTTTTTTGTCGATAAATGACTTAACGTTGATGATCTCTTTAGCA
GAATCTTTGGCTCTAGCTGCAGCTTCTGTTGGTGACAATGGTTGTATGTAAAATCTATCT
TTCAATGCCAATGAAAAGTCTCTTGCTTGGTCTGAGTTATCTGTTCCAGCTGCTCACACA
CAAAAAAACAAAAACAAAAGCAGAACCATAATCAATCAAACAGAACCGGTCTCTATAATC
TAATTACTAAAACTTTTATCTTACAAAAGTGATTAAAGATACGTTTGATAAAAACCTAAA
TTATAACCATGCAAGCTATAACACAATAATTTCGTTTTGTTATCTCCTTAACCAAAAAAA
TAGTATTAGTCATTATGTGAAATTTAATTAAATTAGTTTTACTTACGTAGGCCACCGGAA
GGAAGTGGAGGACCACCAACTTTGATCGGAATAGCTTCGGCGAAAACAGCTTTAACGAAT
GAACCACCGGCTAAACCAGCCGCCACGAGTCCAATCACTGACCGGCGACTACTTTCTGGT
ACTGACACGTTCTGCTGAGCCCTGATCACAAGTCCGGTTCTCTGAGACCCAACCGAGACT
CTTGAGATGTTCAAACGGTTTGAGCCGTTGATCTGTAAACTTCCTTCAAGGACAGCCTGA
GATGCTCCACGTAAGCCAGCCATCGAAGTCACTGCTTATCAGCATGTCCGGGAAATGGCT
TCATGTCCGGGGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAGA
AAGAGTAATTA
   
Sequence Name 50-K117750-0022-424-A06C-8409
GenBank Accession LN507304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117750-0022-424-A06C-8409
CGGACTGCTGATAGCAGTGACTTCGATGGCTGGCTTACGTGGAGCATCTCAGGCTGTCCT
TGAAGGAAGTTTACAGATCAACGGCTCAAACCGTTTGAACATCTCAAGAGTCTCGGTTGG
GTCTCAGAGAACCGGACTTGTGATCAGGGCTCAGCAGAACGTGTCAGTACCAGAAAGTAG
TCGCCGGTCAGTGATTGGACTCGTGGCGGCTGGTTTAGCCGGTGGTTCATTCGTTAAAGC
TGTTTTCGCCGAAGCTATTCCGATCAAAGTTGGTGGTCCTCCACTTCCTTCCGGTGGCCT
ACGTAAGTAAAACTAATTTAATTAAATTTCACATAATGACTAATACTATTTTTTTGGTTA
AGGAGATAACAAAACGAAATTATTGTGTTATAGCTTGCATGGTTATAATTTAGGTTTTTA
TCAAACGTATCTTTAATCACTTTTGTAAGATAAAAGTTTTAGTAATTAGATTATAGAGAC
CGGTTCTGTTTGATTGATTATGGTTCTGCTTTTGTTTTTGTTTTTTTGTGTGTGAGCAGC
TGGAACAGATAACTCAGACCAAGCAAGAGACTTTTCATTGGCATTGAAAGATAGATTTTA
CATACAACCATTGTCACCAACAGAAGCTGCAGCTAGAGCCAAAGATTCTGCTAAAGAGAT
CATCAACGTTAAGTCATTTATCGACAAAAAAGCTTGGCCCTATGTTCAGAACGATCTCCG
TTTAAGAGCATCGTACCTCCGTTACGATCTCAACACCGTTATCTCCGCTAAGCCTAAGGA
AGAAGAGCAAAGCCTTAAAGATCTCACCGCAAAGCTTTTCCAACCATTGACAACG
Primers used for PCR 73vm - 8474, show primer details
   
Sequence Name 44-K117776-0022-424-A06C-73vm
GenBank Accession LN505324 [GenBank]
Sequencing primer gene specific primer, 73vm: 5'-CCAAACTTAGGAAGAATCTTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117776-0022-424-A06C-73vm
CTCTACTTCTTTCCAGAACCGCTAGAAAACAAAACCCTAAAGAAAGAAAAAACTTACGTT
GTCAATGGTTTGGAAAAGCTTTGCGGTGAGATCTTTAAGGCTTTGCTTCTCTTCCTTAGG
CTTAGCGGAGATAACGGTGTTGAGATCGTAACGGAGGTACGATGCTCTTAAACGGAGATC
GTTCTGAACATAGGGCCAAGCTTTTTTGTCGATAAATGACTTAACGTTGATGATCTCTTT
AGCAGAATCTTTGGCTCTAGCTGCAGCTTCTGTTGGTGACAATGGTTGTATGTAAAATCT
ATCTTTCAATGCCAATGAAAAGTCTCTTGCTTGGTCTGAGTTATCTGTTCCAGCTGCTCA
CACACAAAAAAACAAAAACAAAAGCAGAACCATAATCAATCAAACAGAACCGGTCTCTAT
AATCTAATTACTAAAACTTTTATCTTACAAAAGTGATTAAAGATACGTTTGATAAAAACC
TAAATTATAACCATGCAAGCTATAACACAATAATTTCGTTTTGTTATCTCCTTAACCAAA
AAAATAGTATTAGTCATTATGTGAAATTTAATTAAATTAGTTTTACTTACGTAGGCCACC
GGAAGGAAGTGGAGGACCACCAACTTTGATCGGAATAGCTTCGGCGAAAACAGCTTTAAC
GAATGAACCACCGGCTAAACCAGCCGCCACGAGTCCAATCACTGACCGGCGACTACTTTC
TGGTACTGACACGTTCTGCTGAGCCCTGATCACAAGTCCGGTTCTCTGAGACCCAACCGA
GACTCTTGAGATGTTCAAACGGTTTGAGCCGTTGATCTGTAAACTTCCTTCAAGGACAGC
CTGAGATGCTCCACGTAAGCCAGCCATCG
   
Sequence Name 20-K117775-0022-424-A06C-8409
GenBank Accession LN495396 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117775-0022-424-A06C-8409
TCGGACTGAGCCTTTCCCGGACATGCTGATAAGCAGTGACTTCGATGGCTGGCTTACGTG
GAGCATCTCAGGCTGTCCTTGAAGGAAGTTTACAGATCAACGGCTCAAACCGTTTGAACA
TCTCAAGAGTCTCGGTTGGGTCTCAGAGAACCGGACTTGTGATCAGGGCTCAGCAGAACG
TGTCAGTACCAGAAAGTAGTCGCCGGTCAGTGATTGGACTCGTGGCGGCTGGTTTAGCCG
GTGGTTCATTCGTTAAAGCTGTTTTCGCCGAAGCTATTCCGATCAAAGTTGGTGGTCCTC
CACTTCCTTCCGGTGGCCTACGTAAGTAAAACTAATTTAATTAAATTTCACATAATGACT
AATACTATTTTTTTGGTTAAGGAGATAACAAAACGAAATTATTGTGTTATAGCTTGCATG
GTTATAATTTAGGTTTTTATCAAACGTATCTTTAATCACTTTTGTAAGATAAAAGTTTTA
GTAATTAGATTATAGAGACCGGTTCTGTTTGATTGATTATGGTTCTGCTTTTGTTTTTGT
TTTTTTGTGTGTGAGCAGCTGGAACAGATAACTCAGACCAAGCAAGAGACTTTTCATTGG
CATTGAAAGATAGATTTTACATACAACCATTGTCACCAACAGAAGCTGCAGCTAGAGCCA
AAGATTCTGCTAAAGAGATCATCAACGTTAAGTCATTTATCGACAAAAAAGCTTGGCCCT
ATGTTCAGAACGATCTCCGTTTAAGAGCATCGTACCTCCGTTACGATCTCAACACCGTTA
TCTCCGCTAAGCCTAAGGAAGAGAAGCAAAGCCTTAAAGATCTCACCGCANAGCTTTTCC
AAACCATTGACAACGTAAGTTTTTTCTTTCTTTAGGGTTTTGTTTTCTAGCGGTTTCTTG
TTAAAGAAGTTAGAGTGTGCATTGATTTTTTTTATGC
Primers used for PCR 59vn - 8474, show primer details
   
Sequence Name 21-K117967-0022-424-A06C-59vn
GenBank Accession LN495848 [GenBank]
Sequencing primer gene specific primer, 59vn: 5'-TTCTTTTTGGAATGAAATATGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117967-0022-424-A06C-59vn
GATACCAATATTGTTGAACGGTAAGATTGTCTACTTTGGATGCTAAACATGATTAGGTAT
TTGAATTCGTGAATAAGTGACTATTATTCCTAACCCACCCGAAATTAAAAATGTGGATCA
TATAAACTTTAATAGATTGAATTGACCAAATTATTCTTAATGGCTATGTACAAAATCCAA
AATTGAAATTGAGGCCAAAATTATCTATTTTTCAATTTGTACTTTTTTTTAACAATAAGG
GATTTCATTTATTTGTCAAGTTGGATTGGATTTAAGTTACAATTTGCTTAGATATTATCG
AAACCGGTTTTCAATTAACTTTGACTAGGTTTAACCGAATTGGTCAATTTCCACGTCATT
CAGCCACGTGACAATCGCATTCTGGGAACACTCGATCTCAATCCTATGTGGCAAAACTGA
GAATCAAGAAACCACGTGTCAACATATTAGTAGTTAAAACCATATCCGAGTAATCTAAGT
AACAACGTGAATCAGATAAGGTATTCTTTGTCACATAACCAAACACCAAAAATTATAACT
CAACACTTCACTAATCAATTCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGA
CTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACGCTTGTCTACTCC
AAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGG
GTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAG
ATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATC
GTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATC
GTGGAAAAAGAGACGTTCAACCACGTCTTCAAGCAAGTGGATTGATGTGATATATTCATT
GTAAATGGCTTCATGTCCGGGAATCT
   
Sequence Name 05-K117966-0022-424-A06C-8409
GenBank Accession LN488542 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117966-0022-424-A06C-8409
TTACAATTGAATATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCT
TTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCA
TCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTT
TCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCG
GATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCTGAGACTGTATCTTTG
ATATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTG
TCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTTACGAATTGATTAGTG
AAGTGTTGAGTTATAATTTTTGGTGTTTGGTTATGTGACAAAGAATACCTTATCTGATTC
ACGTTGTTACTTAGATTACTCGGATATGGTTTTAACTACTAATATGTTGACACGTGGTTT
CTTGATTCTCAGTTTTGCCACATAGGATTGAGATCGAGTGTTCCCAGAATGCGATTGTCA
CGTGGCTGAATGACGTGGAAATTGACCAATTCGGTTAAACCTAGTCAAAGTTAATTGAAA
ACCGGTTTCGATAATATCTAAGCAAATTGTAACTTAAATCCAATCCAACTTGACAAATAA
ATGAAATCCCTTATTGTTAAAAAAAAGTACAAATTGAAAAATAGATAATTTTGGCCTCAA
TTTCAATTTTGGATTTTGTACATAGCCATTAAGAATAATTTGGTCAATTCAATCTATTAA
AGTTTATATGATCCACATTTTTAATTTCGGGTGGGTTAGGAATAATAGTCACTTATTCAC
GAATTCAAATACCTAATCATGTTTAGCATCCAAAGTAGACAATCTTAACCGTTCAACATA
TTGGTATCACTACTTCATTTTGGTATCTAAATAT

Last Updated on 06.12.2018 16:11