Sequences from confirmation amplicon


Confirmation sequences for line 424B11 and hit At1g48970 are shown.
Line ID 424B11
Locus At1g48970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18116808
Primers used for PCR 21us - 8474, show primer details
   
Sequence Name 56-K118051-0022-424-B11C-21us
GenBank Accession LN509229 [GenBank]
Sequencing primer gene specific primer, 21us: 5'-CTAATGGAGGCTTTGATTTGACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118051-0022-424-B11C-21us
GAACTGAAACAAATTGCGACACGTCTGCTTCTCAGGGAAAAGAAGAACGCCGAAAGTTTC
TGGTATGTATACGAATCTATCAATCTATTTAAGTGTCTCTTGAATAAGCTCTTTGTATGG
TTGAGTTTTGTATTATCCAAAAGAGACCTTTGTGGTGAATTGTGATCCCCAAGTAACATC
GGATGAGGCAGCCTGTAGTGCCGTAGGGACTGGGTAGGCCCGGATATTAATATCTTCTTT
GGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCG
   
Sequence Name 20-K118050-0022-424-B11C-8409
GenBank Accession LN495404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118050-0022-424-B11C-8409
AAAGAAGAAATTAATATCCGGGCCTACCCAGTCCCTACGGCACTACAGGCTGCCTCATCC
GATGTTACTTGGGGATCACAATTCACCACAAAGGTCTCTTTTGGATAATACAAAACTCAA
CCATACAAAGAGCTTATTCAAGAGACACTTAAATAGATTGATAGATTCGTATACATACCA
GAAACTTCGGCGATCTTCTTTTTCCCTTGAGAAGCAGACGTGGTCGCAATTTTGGTTTCA
GATTCCTGAGGAGCTCTAACAGCTAGAGTCAAATCAAAGCCCTCCATTA

Last Updated on 06.12.2018 16:11