Sequences from confirmation amplicon


Confirmation sequences for line 424C01 and hit At2g45290 are shown.
Line ID 424C01
Locus At2g45290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18673324
Primers used for PCR 23qw - 8474, show primer details
   
Sequence Name 42-K115837-0022-424-C01C-23qw
GenBank Accession LN504586 [GenBank]
Sequencing primer gene specific primer, 23qw: 5'-AAGAAAGATAACAATAAAAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115837-0022-424-C01C-23qw
GTCACAATCTCATTGTCAGGTTTGTAAATCTAGCAGCCAAATGCTTCTCCGCAAGAGCTA
AACCAACAGCATTTGCAATACCTTGTCCAAGAGGACCTGCATGTTGGCAAATACAAGCAA
TGCTATATTACAAAAGATCAACATCAACTAAAACACACAAAAGAAGCAGAGAAAATCTAA
CTAACCAGTAGTGGCTTCAACACCAGGAGTCTCAAAATTCTCAGGATGTCCTGGAGTCTT
ACTTCCCCATTGACGGAAGCTCTTCAAATCTTCCTCCTAATAAAAAACTTCAACACTGCT
TCAGCAAATCTTAAGTTGGTTCTACAATTCTAAATCTCAAGACTCCAAAAATCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 10-K115833-0022-424-C01C-8409
GenBank Accession LN490802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115833-0022-424-C01C-8409
TTGATTTTTGGAGTCTTGAGATTTAGAATTGTAGAACCAACTTATGATTTGCTGAAGCAG
TGTTGAAGTTTTTTATTAGGAGGAAGATTTGAAGAGCTTCCGTCAATGGGGAAGTAAGAC
TCCAGGACATCCTGAGAATTTTGAGACTCCTGGTGTTGAAGCCACTACTGGTTAGTTAGA
TTTTCTCTGCTTCTTTTGTGTGTTTTAGTTGATGTTGATCTTTTGTAATATAGCATTGCT
TGTATTTGCCAACATGCAGGTCCTCTTGGACAAGGTATTGCAAATGCTGTTGGTTTAGCT
CTTGCGGAGAAGCATTTGGCTGCTAGATTTAACAAACCTGACAATGAGATTGTTGACCAC
TACACGTAAGATTTTGACTCCTTCAACGGCACTTTTATTGTAATCTTTCT

Last Updated on 06.12.2018 16:11