Sequences from confirmation amplicon


Confirmation sequences for line 424F01 and hit At3g06400 are shown.
Line ID 424F01
Locus At3g06400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1941720
Primers used for PCR BE75 - 8474, show primer details
   
Sequence Name 31-K029490-022-424-F01C-8409
GenBank Accession LN500031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K029490-022-424-F01C-8409
ATGAGCCATTTACAATTGAAGTATCTTCTGCAGCAAACTGAGTTATTTGCCCACTTTGCT
AAAAGTGATGGATCTTCTTCTCAGAAGAAGGCAAAAGGAAGGTGTGTTAGATGCAATTTT
TTCCTAAAATATCGGTTCATCATTGCTTCTAGCTTCAGAGTAAATATGATGAGCCAGGCC
CTGATTTATGTGGTTTTAACTTCTACCTTCAGGGGACGTCATGCTTCCAAAATAACTGAA
GAGGAGGAAGACGAAGAGTATCTAAAGGAAGAAGAGGATGGCTTAACTGGATCTGGAAAC
ACACGGTTACTCACACAGCCCTCTTGTAAGTACCTGTATTTTGCTCTTTAGTCTAGGATT
ACTGTATATTAATCGTCTCTCTAGTCTCGAGCAACAAGTACAATATGATTGACATCTCCG
TCCATTTGCGTAACCATACATATTTTTGTGACCTCACAGTTAGTATGTAGTATAGCGTAA
ATTACTGGGAAAGCTTGAGATTCATCTATTTTTCTTCTCAATCGACAGGTATTCAAGGGA
AGATGAGAGATTACCAATTAGCTGGTTTGAACTGGCTCATTCGTCTTTATGAGAATGGCA
TAAATGGAATTCTTGCTGATGAAATGGTAAATAAT
   
Sequence Name 32-K029490-022-424-F01C-BE75
GenBank Accession LN500454 [GenBank]
Sequencing primer gene specific primer, BE75: 5'-GTTGGGGGAGAATTATTTACCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K029490-022-424-F01C-BE75
ATCATTTATGCCATTCTCATAAAGACGAATGAGCCAGTTCAAACCAGCTAATTGGTAATC
TCTCATCTTCCCTTGAATACCTGTCGATTGAGAAGAAAAATAGATGAATCTCAAGCTTTC
CCAGTAATTTACGCTATACTACATACTAACTGTGAGGTCACAAAAATATGTATGGTTACG
CAAATGGACGGAGATGTCAATCATATTGTACTTGTTGCTCGAGACTAGAGAGACGATTAA
TATACAGTAATCCTAGACTAAAGAGCAAAATACAGGTACTTACAAGAGGGCTGTGTGAGT
AACCGTGTGTTTCCAGATCCAGTTAAGCCATCCTCTTCTTCCTTTAGATACTCTTCGTCT
TCCTCCTCTTCAGTTATTTTGGAAGCATGACGTCCCCTGAAGGTAGAAGTTAAAACCACA
TAAATCAGGGCCTGGCTCATCATATTTACTCTGAAGCTAGAAGCAATGATGAACCGATAT
TTTAGGAAAAAATTGCATCTAACACACCTTCCTTTTGCCTTCTTCTGAGAAGAAGATCCA
TCACTTTTAGCAAAGTGGGCAAATAACTCAGTTTGCTGCAGAAGATACTTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11