Genotyping and primer details


Genotyping and primer details for 424F01 - At3g06400
Line ID 424F01
Locus At3g06400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1941720 of line 424F01 Chr3:1941720
   
Insertion allele amplicon 8474 - BE75
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE75: 5'-GTTGGGGGAGAATTATTTACCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1942349
   
wt amplicon (over InsPos) 08fz - BE75
wt amplicon start Chr3:1941301
wt amplicon end Chr3:1942349
wt amplicon size 1049 bp
Locus specific primer BE75: 5'-GTTGGGGGAGAATTATTTACCATT-3'
2nd locus specific primer 08fz: 5'-TTTGAAATCTCAGTGAACTGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11