Sequences from confirmation amplicon


Confirmation sequences for line 424F04 and hit At5g18620 are shown.
Line ID 424F04
Locus At5g18620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6199443
Primers used for PCR EP42 - 8474, show primer details
   
Sequence Name 15-K103004-0022-424-F04C-8409
GenBank Accession LN492928 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103004-0022-424-F04C-8409
GATTTACATTACAAGTAGATCATTCAGAAATGTGGCAATTGGCTTAGTTATTTTCTCTTT
ACATGTTCTTGTTTCTAGGTAAGATGGTTCTCTTAGATAAATTGCTACCTAAGTTGAAGG
ATCGAGATTCAAGGGTTCTGATATTTTCTCAGGTATTCATTAACGCAAACAAATAATTGT
TCCTATCTTTGATTCCTTTTTTCTGAGTGATATCTTTCTTGGTTCTTTGTGCTTATTCCA
ACGATTTTGCTCATCTGTATCAGATGACAAGGCTTTTGGATATTCTCGAGGATTACCTAA
TGTATCGTGGTTACCAGTACTGCCGTATTGATGGAAATACTGGTGGTGACGAACGAGATG
CTTCCATAGAAGCCTATAACAAGCCAGGAGTGNAGAAATTA
   
Sequence Name 63-K103005-0022-424-F04C-EP42
GenBank Accession LN510962 [GenBank]
Sequencing primer gene specific primer, EP42: 5'-AATTTCTCACTTCCTGGCTTGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K103005-0022-424-F04C-EP42
TATTTCCATCAATACGGCAGTACTGGTAACCACGATACATTAGGTAATCCTCGAGAATAT
CCAAAAGCCTTGTCATCTGATACAGATGAGCAAAATCGTTGGAATAAGCACAAAGAACCA
AGAAAGATATCACTCAGAAAAAAGGAATCAAAGATAGGAACAATTATTTGTTTGCGTTAA
TGAATACCTGAGAAAATATCAGAACCCTTGAATCTCGATCCTTCAACTTAGGTAGCAATT
TATCTAAGAGAACCATCTTACCTAGAAACAAGAACATGTAAAGAGAAAATAACTAAGCCA
ATTGCCACATTTCTGAATGATCTACTTGTAATGTAAATCTTACCAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTNTTATAAAATGAAAG
TACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACCTTCAC

Last Updated on 06.12.2018 16:11