Sequences from confirmation amplicon


Confirmation sequences for line 425B09 and hit At3g44370 are shown.
Line ID 425B09
Locus At3g44370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16030980
Primers used for PCR 14qw - 8474, show primer details
   
Sequence Name 88-K115895-0022-425-B09C-14qw
GenBank Accession LN514893 [GenBank]
Sequencing primer gene specific primer, 14qw: 5'-CAAATACTTTGCTGCTTCCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K115895-0022-425-B09C-14qw
ATAATGATAAGAACACAATTCTCAGATTCACATATTCTCTTAGTATTTCTGAAATTACAT
GTCACAAGACCAAACTCATGAGAATAATTTACTTGTTTTTGTTTTTTACCTTGAGTAGAG
TACCAATGCATCCAAGTAATGAGCTTTGCTCTTAGGATCGTCGGGCTCTTTCATGTTTGC
TACTCTTTCAAGGTGTGTGATTCCTTCTGATGTCTTCCCCTGAAGCCATGAGCGAACATT
TCTTTGAGTATTTTCACATCAATGAGAATAAAACGATAAGACGAAAACAAGCTATTTGAG
TATGATGGCTTCATGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATATCCGCA
   
Sequence Name 58-K115894-0022-425-B09C-8409
GenBank Accession LN509795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K115894-0022-425-B09C-8409
TTACATTGAATACATGAAGCCATCATACTCAAATAGCTTGTTTTCGTCTTATCGTTTTAT
TCTCATTGATGTGAAAATACTCAAAGAAATGTTCGCTCATGGCTTCAGGGGAAGACATCA
GAAGGAATCACACACCTTGAAAGAGTAGCAAACATGAAAGAGCCCGACGATCCTAAGAGC
AAAGCTCATTACTTGGATGCATTGGTACTCTACTCAAGGTAAAAAACAAAAACAAGTAAA
TTATTCTCATGAGTTTGGTCTTGTGACATGTAATTTCAGAAATACTAAGAGAATATGTGA
ATCTGAGAATTGTTGTTCTTATCATTATGTGTGCAGTGCGATATTCAACGAAGGAAGGAG
AGAGGAAGCACANAAGTATTTG

Last Updated on 06.12.2018 16:11