Sequences from confirmation amplicon


Confirmation sequences for line 425C12 and hit At2g25600 are shown.
Line ID 425C12
Locus At2g25600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10895677
Primers used for PCR 36vz - 8474, show primer details
   
Sequence Name 53-K118393-0022-425-C12C-36vz
GenBank Accession LN508298 [GenBank]
Sequencing primer gene specific primer, 36vz: 5'-GAAAATACTCTGCTTTCATTTCCG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118393-0022-425-C12C-36vz
TATGTGAATAAAGTGACCACTTTTAGCATGCAGGACTTCTTGCGTTATGGGAAACATACC
AACTGGAAAAGAAGATCATTGGATATTCCATGGAACAAGTAAATTTTGTCAACAACTTCA
TAGAACAGATAATGTGATATGCTGGACCGAATAGCTTTGGGAAGAGAATCGATTATTTCT
TGCTGCTGTAAGCCTTCTGAATCAGTTCTGTACCTCAAACACAAATGAGCAACCATCTGA
TCTTGTAACCGCACTGGCAGATTGTTCCTCTGACCAAACCCTGATGCGGCTTGGATGGTA
TCTCTCTAAAATAATGCAAATGCTTAAGACTCATGAAACTTCACTGAGACCAGACTAATT
TTCAGAGAAGTGCAGTTAACAGTACCGGTACATAGAACTTACAAATTTTCTGGTGCGGCC
GGTCACATGAACCACCAAGTTGGTCATATTACCAATTATATATGCTGACAGTCCAAGATT
AAAAACCATGTAGAAAAGAATGAAAGTCATCTCTCTTGAGTTAACACCATGTATATCACC
ATAACCAGTTGTGGAGAAAGTAGTTATGGCTTCATGTCCGGGATATATTCAATTGTAAAT
GGCTTCATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 05-K118392-0022-425-C12C-8409
GenBank Accession LN488554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118392-0022-425-C12C-8409
CGGACATGAAGCCATAACTACTTTCTCCACAACTGGTTATGGTGATATACATGGTGTTAA
CTCAAGAGAGATGACTTTCATTCTTTTCTACATGGTTTTTAATCTTGGACTGTCAGCATA
TATAATTGGTAATATGACCAACTTGGTGGTTCATGTGACCGGCCGCACCAGAAAATTTGT
AAGTTCTATGTACCGGTACTGTTAACTGCACTTCTCTGAAAATTAGTCTGGTCTCAGTGA
AGTTTCATGAGTCTTAAGCATTTGCATTATTTTAGAGAGATACCATCCAAGCCGCATCAG
GGTTTGGTCAGAGGAACAATCTGCCAGTGCGGTTACAAGATCAGATGGTTGCTCATTTGT
GTTTGAGGTACAGAACTGATTCAGAAGGCTTACAGCAGCAAGAAATAATCGATTCTCTTC
CCAAAGCTATTCGGTCCAGCATATCACATTATCTGTTCTATGAAGTTGTTGACAAAATTT
ACTTGTTCCATGGAATATCCAATGATCTTCTTTTCCAGTTGGTATGTTTCCCATAACGCA
AGAAGTCCTGCATGCTAAAAGTGGTCACTTTATTCACATTAAGTCTTAACTTACGGTGTC
CTGTAGGTCACGGAAATG

Last Updated on 06.12.2018 16:11