Sequences from confirmation amplicon


Confirmation sequences for line 425D06 and hit At5g48180 are shown.
Line ID 425D06
Locus At5g48180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19542376
Primers used for PCR 25fr - 8474, show primer details
   
Sequence Name 41-K111470-0022-425-D06C-25fr
GenBank Accession LN504122 [GenBank]
Sequencing primer gene specific primer, 25fr: 5'-CTTTAGCAGTAACACCACCAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K111470-0022-425-D06C-25fr
ATAGCTACGACCAGGAAGCCCTTCCTCAACAGGAGCCAACAATTTCCACTCATTGGTCTC
AGTATCATACGAATGAAGACCGTTGTATCTCCGTTTATCGTCACGGCCACCGTAGACATA
GATCGTTGAACCGATCGTTACCATCGAGACACCAAAACAGGGGAAAGGAGCCTCTCCGGT
CGCAGGAGCTATCGACCATTCTTGAGTCTCAAGATCGAAAACGTAAAGATCGTTATCAAT
ATGGATCGTTGGCTTAAGCTCACCACCAAAACAGTAAACTTTGTTCCCAACGACGGTCAT
GGCATGTGAGCTTCTAGCCCCAGGACCAGCTCCTTTTTGACCCACCTGATTAACATAATC
ACATTACATTTCTAAGATCAAAGAAACCAAATAAAAGAATCTTAATATGTATTATAGAGA
TTGAAAGATTGAATCTTTACCTTTAACCATTTGTTCTCCACCGGACACATTTTTTTCGTT
ACTCACCATTGGCGGGAAATGCTAATGTTGATGCTAATAAACGCTCTTTTCTCTTAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 25-K111469-0022-425-D06C-8409
GenBank Accession LN497504 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111469-0022-425-D06C-8409
ATATATCCTAAGAGAAAGAGCGTTTATTAGCATCAACATTAGCATTTCCCGCCAATGGTG
AGTAACGAAAAAAATGTGTCCGGTGGAGAACAAATGGTTAAAGGTAAAGATTCAATCTTT
CAATCTCTATAATACATATTAAGATTCTTTTATTTGGTTTCTTTGATCTTAGAAATGTAA
TGTGATTATGTTAATCAGGTGGGTCAAAAAGGAGCTGGTCCTGGGGCTAGAAGCTCACAT
GCCATGACCGTCGTTGGGAACAAAGTTTACTGTTTTGGTGGTGAGCTTAAGCCAACGATC
CATATTGATAACGATCTTTACGTTTTCGATCTTGAGACTCAAGAATGGTCGATAGCTCCT
GCGACCGGAGAGGCTCCTTTCCCCTGTTTTGGTGTCTCGATGGTAACGATCGGTTCAACG
ATCTATGTCTACGGTGGCCGTGACGATAAACGGAGATACAACGGTCTTCATTCGTATGAT
ACTGAGACCAATGAGTGGAAATTGTTGGCTCCTGTTGAGGAAGGGCTTCCTGGTCGTAGC
TATCATTCAATGGCTGGTGATGATCGGAAAGTTTATGTGTTTGGTGGTGTTACTGCTAAA
G

Last Updated on 06.12.2018 16:11