Sequences from confirmation amplicon


Confirmation sequences for line 425G12 and hit At4g16100 are shown.
Line ID 425G12
Locus At4g16100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9105896
Primers used for PCR KP41 - 8474, show primer details
   
Sequence Name 19-K117753-0022-425-G12C-KP41
GenBank Accession LN494943 [GenBank]
Sequencing primer gene specific primer, KP41: 5'-GGGTTTCCTCAATTGTATGAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117753-0022-425-G12C-KP41
TACCATGTCTCCATACTTTGACTATAAATGAATGCAGACAGTTGAAGGAGCTTCCGGATG
AGGTCATATACATAACATCCTTAAAACAACTGACAATTGGAGGAAGGAATAGTGAATGGG
TGTGGAAATTGTCTGAAAGAGGACAAGATCACTACAAAGTTCACCACATTCCTTGTGTTC
AGTTTATTTACTAGACCTTTGTCTAATTTTATGTATAAAAATTATAACAATGTAGAAAAC
TACAACACAAATGAGATCAAATTCTGATTTTTAAAATCCGGTTGTGATGATTATTAATCG
TGGAAAAAAATCAGGGATTATAACTTTTGTTAAATTGTGAACGTACAATATTGAACTGAA
AATGCCAAATGAATTGGTTATTATTACTTTGATGAAAATGAATTGATCGTTTAATTTTTT
TTATATTTATAACAAAAATGCGATATGCTCTTTTTAATTCTTTATTTCTATTTTTGCTTC
AGAATTTTTTTTCATTTTATTTTTGGTCAAAAAGGTTATTAATTTCCTTTTCAATTTGTA
CGAATTTAAACGGTGACGTTTTTGTTTAAATTATCAAACAAATCAAAACAAAATCAATAA
AATCTAATCTCTCATCATGAGGCATCTTTGACTGCTCTTTAATTCACTAAAAAAGTCTAA
AACCCTAACCATTGCCCTTTTTCAACGAAGAAGAAGAAGACGAAACGCTACCATCATCTA
CACACACACACCCAACTACTTTTTTCCATCGCCGGCCTGTACAAATGTCGA
   
Sequence Name 03-K117752-0022-425-G12C-8409
GenBank Accession LN487665 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117752-0022-425-G12C-8409
ACATTGAATTATCCTGAGGAGTTTTCTTCTTCTCACGCTCTTGTTGAAGTTTTCTCATCG
GAGGTGGGTTATAAAACCGATTCTCGCCGCGGATCCTACGGGTAGTCGACATTTGTACAG
GCCGGCGATGGAAAAAAGTAGTTGGGTGTGTGTGTGTAGATGATGGTAGCGTTTCGTCTT
CTTCTTCTTCGTTTGAAAAAGGGCAATGGTTAGGGTTTTAGACTTTTTTAGTGAATTAAA
GAGCAGTCAAAGATGCCTCATGATGAGAGATTAGATTTTATTGATTTTGTTTTGATTTGT
TTGATAATTTAAACAAAAACGTCACCGTTTAAATTCGTACAAATTGAAAAGGAAATTAAT
AACCTTTTTGACCAAAAATAAAATGAAAAAAAATTCTGAAGCAAAAATAGAAATAAAGAA
TTAAAAAGAGCATATCGCATTTTTGTTATAAATATAAAAAAAATTAAACGATCAATTCAT
TTTCATCAAAGTAATAATAACCAATTCATTTGGCATTTTCAGTTCAATATTGTACGTTCA
CAATTTAACAAAAGTTATAATCCCTGATTTTTTTCCACGATTAATAATCATCACAACCGG
ATTTTAAAAATCAGAATTTGATCTCATTTGTGTTGTAGTTTTCTACATTGTTATAATTTT
TATACATAAAATTAGACAAAGGTCTAGTAAATAAACTGAACACAAGGAATGTGGTGAACT
TTGTAGTGATCTTGTCCTCTTTCAGACAATTCCACACCCATTCACTA

Last Updated on 06.12.2018 16:11