Sequences from confirmation amplicon


Confirmation sequences for line 426C05 and hit At2g28500 are shown.
Line ID 426C05
Locus At2g28500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12187015
Primers used for PCR 47pv - 8474, show primer details
   
Sequence Name 46-K115234-0022-426-C05C-47pv
GenBank Accession LN505954 [GenBank]
Sequencing primer gene specific primer, 47pv: 5'-GCAGTTTCGTTACGTGCTTATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115234-0022-426-C05C-47pv
CAACTCGCATATATCATATCCTAGGTCTTCATATGCATATGGAAGTAACAACTAAAGCGC
TTTAAAGTGAGCTAATAATACCATAAGTTATGATCATGTCCAAAGAGGATCCCACCACGA
CATTGATGAATTATTGTTGTTGTTGTTGTCCTCAAGGAACCCCAAATCATTGCTCTCTTC
GTCCGGGCTACTAATGAACTCATCGCCGCTTTCAAATGAACTCTCAAAGGACATCTTCTG
TTGTCCTTGCTCTTGTACTGACAACTTTGTTTGCTCCATATTATATATCAATTCTAGTAG
ACTTGATCTTTGGAGTTGCATGCCTACTAACTCTACTTGAGTTTTTGCAAGTTGTGCTTG
AAGCTCACTCACTTGTCTCTGCAAATGATAAATTGCACCCGCACATCCNTAAACCGGATC
TCTCATCCTAGCTCCGGCTTCATAGACCATGCTATTAACAGCATCTGTTCTTTGCGATTC
TGGAAGTTCCTGATTATAATAAATATCCGGGAAATATCAATTTGCTGATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCAGCAGCGT
TATA
   
Sequence Name 22-K115233-0022-426-C05C-8409
GenBank Accession LN496239 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115233-0022-426-C05C-8409
TTGAATATCAGCAAATTGATATTTCCCGGATATTTATTATAATCAGGAACTTCCAGAATC
GCAAAGAACAGATGCTGTTAATAGCATGGTCTATGAAGCCGGAGCTAGGATGAGAGATCC
GGTTTACGGATGTGCGGGTGCAATTTATCATTTGCAGAGACAAGTGAGTGAGCTTCAAGC
ACAACTTGCAAAAACTCAAGTAGAGTTAGTAGGCATGCAACTCCAAAGATCAAGTCTACT
AGAATTGATATATAATATGGAGCAAACAAAGTTGTCAGTACAAGAGCAAGGACAACAGAA
GATGTCCTTTGAGAGTTCATTTGAAAGCGGCGATGAGTTCATTAGTAGCCCGGACGAAGA
GAGCAATGATTTGGGGTTCCTTGAGGACAACAACAACAACAATAATTCATCAATGTCGTG
GTGGGATCCTCTTTGGACATGATCATAACTTATGGTATTATTAGCTCACTTTAAAGCGCT
TTAGTTGTTACTTCCATATGCATATTGAAGACCTAGGATATGATATATGCGTAGTTTGTT
TTTTTCTTAATTTCTCTAAGTCAACCAATTATGTATAAGCACGTAAGNAAAACTG

Last Updated on 06.12.2018 16:11