Sequences from confirmation amplicon


Confirmation sequences for line 427C03 and hit At5g58870 are shown.
Line ID 427C03
Locus At5g58870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23770837
Primers used for PCR BC05 - 8474, show primer details
   
Sequence Name 39-K103981-0022-427-C03C-BC05
GenBank Accession LN503331 [GenBank]
Sequencing primer gene specific primer, BC05: 5'-GAGTCTAGTGACCATATCATGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103981-0022-427-C03C-BC05
CCCTCTCAGAGATTATAAGCCCAACTAGCCGTTCTTTAGAATACTGGTCTTATGTTTTGT
TTCAGTAATTATAGTTTTAGTATCCAGAGGTGTCATGTGTTACCTTCCAGTTGAGCACCG
AGCCCTTCTAATACATCTGGATTAGCGCGAACAACAGTCAACGCAACATCCAGAGCAGAT
TGCAACAAATTTGTCACCTCTCTTTGAACAAGGTCAACCAGATGCCCCTGTAAATTCAGC
ATGACATGTTAAGTTTGCTTTCCATTTTGAAAAAGACCAACAAGAAGTTTAGGTAGAATA
GAGTTACCTGATCTCTTCCCCATGGTGATCCCCCAGAGTCATCAATCCCACCAGCAGAAA
GCGTAGCCACAGAGACGGGTCCGATTTTCTCGTTGAGACCATATTCAGCTACTGCCTTAT
AGGCCATGTCGGTTGCTCTCCTGATATCATCAAGTGCACCTGTTGATATACGCCCCGAGT
AAACAACCTCTTCATGAATAAAATGTACATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATT
   
Sequence Name 55-K103982-0022-427-C03C-8409
GenBank Accession LN508792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103982-0022-427-C03C-8409
TTCGGACTGAGCCTGTCATTTTATTCATGAAGAGGTTGTTTACTCGGGGCGCTATATCAA
CAGGTGCACTTGATGATATCAGGAGAGCAACCGACATGGCCTATAAGGCAGTAGCTGAAT
ATGGTCTCAACGAGAAAATCGGACCCGTCTCTGTGGCTACGCTTTCTGCTGGTGGGATTG
ATGACTCTGGGGGATCACCATGGGGAAGAGATCAGGTAACTCTATTCTACCTAAACTTCT
TGTTGGTCTTTTTCAAAATGGAAAGCAAACTTAACATGTCATGCTGAATTTACAGGGGCA
TCTGGTTGACCTTGTTCAAAGAGAGGTGACAAATTTGTTGCAATCTGCTCTGGATGTTGC
GTTGACTGTTGTTCGCGCTAATCCAGATGTATTAGAAGGGCTCGGTGCTCAACTGGAAGG
TAACACATGACACCTCTGGATACTAAAACTATAATTACTGAAACAAAACATAAGACCAGT
ATTCTAAAGAACGGCTAGTTTGGGCTTATAATCTCTGAGATGGGCTGGTTCATTCCAATG
GTATCAAAACAACATGGCATGATATG

Last Updated on 06.12.2018 16:11