Sequences from confirmation amplicon


Confirmation sequences for line 428E05 and hit At1g10560 are shown.
Line ID 428E05
Locus At1g10560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3486030
Primers used for PCR BB79 - 8474, show primer details
   
Sequence Name 08-K104123-0022-428-E05C-BB79
GenBank Accession LN489706 [GenBank]
Sequencing primer gene specific primer, BB79: 5'-GACACAAGTTCAAAATCAGACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K104123-0022-428-E05C-BB79
GGAGATCCTCCGACGAAGAAAAATCTTCACCGCGAGTTTCAAACCGCCACGACGAATCAC
CCCAATCGTCCCATCAGGATACTCCGCCAGCTTCGCCAACGTCGCTAAACAATCCGCCGT
GAGTCCGCCGCTAATCTCCCCCGATCTCAACAGATCAAGAAGAATCGGAACAGCTCCGGC
GGCGAGAACACGCCAATGGTTATCGGATTGCATTAACAAACCCATAACCGCAAGCAACGC
GCTACGTTTGGCGGAATCGCCGTAATCATCTCCTTTAACGATATTCATCAATCCCGGAAT
CGCATCTGGATTTTCTCCGATCAATCTGCTGTAATCTTCAACGGAAGAGAGATAAAACAG
AGCAGAAGCAGAGTATAGTCTCGTCTCTGTTTTAGCTCCTTCGTTGAGAATCTCTACAAG
TATCTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K104124-0022-428-E05C-8409
GenBank Accession LN496966 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K104124-0022-428-E05C-8409
GCCATGTAGATACTTGTAGAGATTCTCAACGAAGGAGCTAAAACAGAGACGAGACTATAC
TCTGCTTCTGCTCTGTTTTATCTCTCTTCCGTTGAAGATTACAGCAGATTGATCGGAGAA
AATCCAGATGCGATTCCGGGATTGATGAATATCGTTAAAGGAGATGATTACGGCGATTCC
GCCAAACGTAGCGCGTTGCTTGCGGTTATGGGTTTGTTAATGCAATCCGATAACCATTGG
CGTGTTCTCGCCGCCGGAGCTGTTCCGATTCTTCTTGATCTGTTGAGATCGGGGGAGATT
AGCGGCGGACTCACGGCGGATTGTTTAGCGACGTTGGCGAAGCTGGCGGAGTATCCTGAT
GGGACGATTGGGGTGATTCGTCGTGGCGGTTTGAAACTCGCGGTGAAGATTTTGTCTTCG
TCGGAGGATTCTCCGGTGGCGGTGAAGCAGCATTGTGTTGGTCTGATTTTGACCTTGTGT
C

Last Updated on 06.12.2018 16:11