Sequences from confirmation amplicon


Confirmation sequences for line 429H05 and hit At1g36280 are shown.
Line ID 429H05
Locus At1g36280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13642637
Primers used for PCR 55ra - 8474, show primer details
   
Sequence Name 70-K118684-0022-429-H05C-55ra
GenBank Accession LN512471 [GenBank]
Sequencing primer gene specific primer, 55ra: 5'-AACAAGCAGAATCATCTACACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118684-0022-429-H05C-55ra
CATCATCCACGGCTAGTGCCAATGCAGCAGCAGCGCCAACATATGTGTGTGGAGTTAGCT
TCAAAAGTTGGTCTTTTGCTTCTTCAGGCAATTCCAAACTTTTAATAAACTTTCTAATGC
TTTCTTCATTCACAGCTCTTCCTCTTGTTAGCTCCTTCAGCTTCTCATACGGCTCTGGAA
CACCATATCTCCTCATCACCTGTTTTTTTTATTCCTTCAGTTACCAATGTACATGGAATA
ATAACTTGATCATTATAATAAGACTAGAAAGTTTCGAGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 58-K118683-0022-429-H05C-8409
GenBank Accession LN509838 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118683-0022-429-H05C-8409
TGATCAAGTTATTATTCCATGTACATTGGTAACTGAAGGAATAAAAAAAACAGGTGATGA
GGAGATATGGTGTTCCAGAGCCGTATGAGAAGCTGAAGGAGCTAACAAGAGGAAGAGCTG
TGAATGAAGAAAGCATTAGAAAGTTTATTAAAAGTTTGGAATTGCCTGAAGAAGCAAAAG
ACCAACTTTTGAAGCTAACTCCACACACATATGTTGGCGCTGCTGCTGCATTGGCACTAG
CCGTGGATGATGCTGTGCACTTGGGACATTAATAATATGATCATATTGGTGTAGATGATC
NTGCTTGTT

Last Updated on 06.12.2018 16:11