Sequences from confirmation amplicon


Confirmation sequences for line 430G11 and hit At2g41730 are shown.
Line ID 430G11
Locus At2g41730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17409653
Primers used for PCR CB31 - 8474, show primer details
   
Sequence Name 01-K104526-0022-430-G11C-CB31
GenBank Accession LN486563 [GenBank]
Sequencing primer gene specific primer, CB31: 5'-GACTTTAAGATTTGTAACCGCGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104526-0022-430-G11C-CB31
CTTCCTTACTTGCTCGTTTGGTTGTGGACCGGCTTATTGCTCTCAAGTCTCAATCGTTGG
GAAGAAGTTGATGCCTTGATGGTGTTCTCAATTCCTGGGCTGGCCGGTTTGCTAGAAAGA
ATTTCCCGGTTCTTATCAAGGTACGTATGTTTGATTTTGATTGGTATTCACTGAGTGTGT
TTTGAAACTAAAAAATTTGCAATGACAAAAAAGTATTTCTTCCCATAATTAATATCATTG
GTTATCAAAGTTCAAAACATAAAAGAAAAAAACTATAATCATCATACATCTCAAACAAAA
TGATTTAATCAATGTTCATCATGATCTTCACCACATAATGTATCCAAAATATCTTTAGCA
GAGGACGAGTCCGACCCGATGAGCCGGTCTAGCTTATCCTCACTCCACCAACTTGTAGGT
TGTAGCTTACGGCGACCCTTAGATTTGTGGCCCGACTTGATAGCTGGCGACACGTGACAA
TGACTGTACTTGGACCGCTCGCTGGTGGTATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 17-K104527-0022-430-G11C-8409
GenBank Accession LN493839 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104527-0022-430-G11C-8409
GCCATTTACAATTGAATATACCACCAGCGAGCGGTCCAAGTACAGTCATTGTCACGTGTC
GCCAGCTATCAAGTCGGGCCACAAATCTAAGGGTCGCCGTAAGCTACAACCTACAAGTTG
GTGGAGTGAGGATAAGCTAGACCGGCTCATCGGGTCGGACTCGTCCTCTGCTAAAGATAT
TTTGGATACATTATGTGGTGAAGATCATGATGAACATTGATTAAATCATTTTGTTTGAGA
TGTATGATGATTATAGTTTTTTTCTTTTATGTTTTGAACTTTGATAACCAATGATATTAA
TTATGGGAAGAAATACTTTTTTGTCATTGCAAATTTTTTAGTTTCAAAACACACTCAGTG
AATACCAATCAAAATCAAACATACGTACCTTGATAAGAACCGGGAAATTCTTTCTAGCAA
ACCGGCCAGCCCAGGAATTGAGAACACCATCAAGGCATCAACTTCTTCCCAACGATTGAG
ACTTTGAGAGCAATAAGCCGGTCCACAACCAAACGAGCAAGTAAGGAAGGCTCTTCTGTG
GATAAAAGGATAACTTTACTCGCGGTTAC

Last Updated on 06.12.2018 16:11