Sequences from confirmation amplicon


Confirmation sequences for line 430G11 and hit At4g38050 are shown.
Line ID 430G11
Locus At4g38050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17871813
Primers used for PCR RU64 - 8474, show primer details
   
Sequence Name 19-K026865-022-430-G11C-8409
GenBank Accession LN494609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K026865-022-430-G11C-8409
GTAGATTTCCCGGACATGAAGCCATATACTCATTTAAAGGAAGATTCTTTTTGTTGAATT
TGAAATTGCAGTTCCACTTATCTATTACGGTCTGCAACACTATCTGTCACTTGTTGGTTC
ACTTGTGTTTATTCCTCTGGTCATTGTACCAGCCATGGATGGTTCCGATGTAAGCTTCTT
CTTCTTCTTCCATGGATTATTCTGTGAGAACTTCTTTGATTAGTAATTGACTTTTTATGA
TGGTAGAAAGATACAGCCTCAGTGATTTCAACAATGCTGCTTCTTACTGGAGTTACAACT
ATACTTCACTGTTACTTCGGTACTCGGCTTCCGTTGGTTCAAGGAAGCTCTTTTGTTTAC
TTGGCCCCGGTTTTGGTTGTCATCAACTCTGAGGAGTTCAGAAACCTCACTGAGCATGTA
AGTTTGCATATATAGAAATCAAAAGGAGGATTTTGAGTTCTTGGAGACTTTGCAATAAAC
TGATGTTTTAGCT
   
Sequence Name 20-K026865-022-430-G11C-RU64
GenBank Accession LN495062 [GenBank]
Sequencing primer gene specific primer, RU64: 5'-AATTTCTGTGCGAAGCTAAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K026865-022-430-G11C-RU64
AGTTTATTGCAAAGTCTCCAAGAACTCAAAATCCTCCTTTTGATTTCTATATATGCAAAC
TTACATGCTCAGTGAGGTTTCTGAACTCCTCAGAGTTGATGACAACCAAAACCGGGGCCA
AGTAAACAAAAGAGCTTCCTTGAACCAACGGAAGCCGAGTACCGAAGTAACAGTGAAGTA
TAGTTGTAACTCCAGTAAGAAGCAGCATTGTTGAAATCACTGAGGCTGTATCTTTCTACC
ATCATAAAAAGTCAATTACTAATCAAAGAAGTTCTCACAGAATAATCCATGGAAGAAGAA
GAAGAAGCTTACATCGGAACCATCCATGGCTGGTACAATGACCAGAGGAATAAACACAAG
TGAACCAACAAGTGACAGATAGTGTTGCAGACCGTAATAGATAAGTGGAACTGCAATTTC
AAATTCAACAAAAAGAATCTTCCTTTAAATGAGTATATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA

Last Updated on 06.12.2018 16:11