Sequences from confirmation amplicon


Confirmation sequences for line 433D08 and hit At5g54040 are shown.
Line ID 433D08
Locus At5g54040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21929744
Primers used for PCR 40xt - 8474, show primer details
   
Sequence Name 43-K119432-0022-433-D08C-40xt
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 40xt: 5'-GATGTACTTCCATCCTAGGACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K119432-0022-433-D08C-40xt
ATCCCCAGTACACAATCAATATGGAGACTTAGCCCACAATCATCACAAGTATAGTAAAAN
TACACTCGGGTCTGTCTTTGTCTCACAAATATCACACCACAAGTCACCACTTGCACTTCC
CACACCTTGACATAGTGACAGATAATGATCATCACATCTGTGTTTTACCTTTCTTGGTAA
AGCAGCGCACCTCGTGCATAAAGAGTAATCATCACAAACCGTACAATTCAAGGGATTCCA
ATCTATTTTTCTTTTACAAGCGTGACATTTATTGGTTTTCTCCAACGTAAAATATAAGGG
ATGTTGATGCAGATCATGGTGAAACGGTTCCGATACAGAACTACACCGAACATCGTACGT
AAAACTAATACTTTTGCAACCTTCATGAAGACACTCGTAAATGAAACCATTAGTTTTTAT
GTCACAAGCTTTACATCTCAAAAAACGGTTACCTGTCGATGAAAGAGTGAGTTTATGCTT
ATGCAATAAATGTCGCATTTTTCGAGGAAGATTCGCACACGCTTTGTGAAGGATGAAATC
ACATTCAACGCATCTGTAGCATGGGTCACCGTTGATAGCAAGAACGCAACACCGACNACG
CAGGTTAGCATCATCCGTTACATAAATCTCGTCCGACAACGCANGTTAGTCATGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCATAAATGTAGATATCCGCA
   
Sequence Name 03-K119431-0022-433-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119431-0022-433-D08C-8409
CATTTCATGACTAAATGCGTTGTCGGACGAGATTTATGTAACGGATGCTGTTAACATGCG
TTGTCGGTGTTGCGTTCTTGCTATCAACGGTGACCCATGCTACAGATGCGTTGAATGTGA
TTTCATCCTTCACAAAGCGTGTGCGAATCTTCCTCGAAAAATGCGACATTTATTGCATAA
GCATAAACTCACTCTTTCATCGACAGGTAACCGTTTTTTGAGATGTAAAGCTTGTGACAT
AAAAACTAATGGTTTCATTTACGAGTGTCTTCATGAAGGTTGCAAAAGTATTAGTTTTAC
GTACGATGTTCGGTGTAGTTCTGTATCGGAACCGTTTCACCATGATCTGCATCAACATCC
CTTATATTTTACGTTGGAGAAAACCAATAAATGTCACGCTTGTAAAAGAAAAATAGATTG
GAATCCCTTGAATTGTACGGTTTGTGATGATTACTCTTTATGCACGAGGTGCGCTGCTTT
ACCAAGAAAGGTAAAACACAGATGTGATGATCATTATCTGTCACTATGTCAAGGTGTGGG
AAGTGCAAGTGGTGACTTGTGGTGTGATATTTGTGAGACAAAGACAGACCCGAGTGTATT
TTACTATACTTGTGATGATTGTGGGCTAAGTCTCCATATTGATTGTGTACTTGGGGATTG
CTATTATACCAAAGTCGGATGCCCAGGTCCTAGGATGGAAGTACATC
Primers used for PCR 47xw - 8474, show primer details
   
Sequence Name 50-K119542-0022-433-D08C-47xw
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 47xw: 5'-TTTATATTAATTAGCCGGCAGTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K119542-0022-433-D08C-47xw
TAAACTAAAATTAATTGATGAGTTCCAGGAAGTTAAGTACTACATAATGAGGAGCTCACA
GGACAAAATTATAACTCAACTTCTGCTTTTTATTTTCTTCTTTGAATATACAAAAATGAC
TCCTGATGATTTCCAACTAGCCACCATGCCTAAACTCACGGTTCCTTTTCATGATCATCC
TATGTCAAGTTCTTATTGTATTTTTTTGGAATCTTGCTCTTTGTGCTCTTTCTCTAAGAA
CTCTTCATGGCTAAGAAGAAGTGTTACTTGTATCAGTTACGAGTGTACGGATTGTGGCTT
GACGTTGCACAAAGAATGCGTTGACCACCTCTTCCACAACCGGCCATTTCTATGCAATCA
CGTTCTCAAGATCTACCGTAATACCTTCGCGTGGCCTGACCCTGACCGTAAAGACGACCT
TTCTTGTCATTTTTGTCGACGTAAGCTTACGTCTTTCTTTGCTCGGCTACGTAATCTTCC
GTCTTTCTTTGCTCGTTGCACGATATGCAACATAAACGTGGATAAAAAATGTTTAATGGC
ATTGGGACCACCACTCGCAATTTTCGAACCAAAACACCACGAGCATAGTCTCACCCTCTT
GTTAAGGTTAGTCACCTTTACTTGTAATGCTTGTGGTGTGGTAGATGACCGCAATCCTTA
CGTATGTCTTGAATGCAATTTGATGGTCCACAAAGAGTGTGTTC
   
Sequence Name 18-K119541-0022-433-D08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119541-0022-433-D08C-8409
ATTTACAATTGAATATATCCTTGAATGAATGACTAAAATGCTCTTCATGTAAATGAATGA
ATGCTCTTCATGACTAAAATGAATGATATCTCCCTTTTCATTAATTACCTTGAATGGATC
TTCGATTTCTTCTTCTTCTTCGGGCACATCTTCGAGCTCTAAACCATCCCACACTTCGTA
TCTTGTTGCACATCTTGAATGAACGGCATAACTAGGACAACGAGAGCATTTATAACCTCC
ATAGACCCAATTGATCGTCTTCCGACAAACTCCACACTCCCAATCTCCTTCTCCTTGACC
AAGGTGAAAAGTATGAGATATGCGATGGTCATGACGATTTATGCTTATGACTCGCGGTAG
ATTCTGAACACACTCTTTGTGGACCATCAAATTGCATTCAAGACATACGTAAGGATTGCG
GTCATCTACCACACCACAAGCATTACAAGTAAAGGTGACTAACCTTAACAAGAGGGTGAG
ACTATGCTCGTGGTGTTTTGGTTCGAAAATTGCGAGTGGTGGTCCCAATGCCATTAAACA
TTTTTTATCCACGTTTATGTTGCATATCGTGCAACGAGCAAAGAAAGACGGAAGATTACG
TAGCCGAGCAAAGAAAGACGTAAGCTTACGTCGACAAAAATGACAAGAAAGGTCGTCTTT
ACGGTCAGGGTCAGGCCACGCGAAGGTATTACGGTAGATCTTGAGAACGTGATTGCATAG
AAATGGCCGGTTGAGGAAGAGGTGGTCAACGCATTCTTTGTGCAACGTCAAGCCACAATC
CGTACACTCGTAACTGATACAAGTAACACTTCTTCTTAGCCATGAAGAGTTCTTAGAGAA
AGAGCACAAAGAGCAA

Last Updated on 06.12.2018 16:11