Sequences from confirmation amplicon


Confirmation sequences for line 434G03 and hit At3g25560 are shown.
Line ID 434G03
Locus At3g25560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9281602
Primers used for PCR EQ68 - 8474, show primer details
   
Sequence Name 41-K103105-0022-434-G03C-EQ68
GenBank Accession LN504040 [GenBank]
Sequencing primer gene specific primer, EQ68: 5'-GTCAAGCTTACACCGAAGACTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103105-0022-434-G03C-EQ68
TGATTTATCTAACAACAACAACAACAACAAGAAGAAAAGAAAAATCAGTTTAGATATATT
CTCTAGGATTCAAATATTACTTTAAAATATTGAAAATTTAGATATTACTTTGAGAACTGT
TCAAGGTGATTGACATTGGCTTAGGCTGAGTCCCATTACAGTCTTTCTCAGTTCCTGTTG
GACAAATCTGAGAATTGCCCATAACACTGCATAACAAGTTAAATAAAAACTATATAATAT
AATTAGGGTAACGTATAAAAGTGGATAAATTACAGAGAAAAGAAAAACACAATTACACTG
TTACTTTCTCAGAACAGAGTAAAACAGAGCAGACAAATATTCTTTCTCGTAAATAGACTT
ACTTGAATGTTTTGGCAAGTGATCTTGGAACTGGTCCACTCAAGTTATTATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCACACGGT
TTATA
   
Sequence Name 41-K103106-0022-434-G03C-8409
GenBank Accession LN504041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103106-0022-434-G03C-8409
ATTTACAATTGAATAATAACTTGAGTGGACCAGTTCCAAGATCACTTGCCAAAACATTCA
AGTAAGTCTATTTACGAGAAAGAATATTTGTCTGCTCTGTTTTACTCTGTTCTGAGAAAG
TAACAGTGTAATTGTGTTTTTCTTTTCTCTGTAATTTATCCACTTTTATACGTTACCCTA
ATTATATTATATAGTTTTTATTTAACTTGTTATGCAGTGTTATGGGCAATTCTCAGATTT
GTCCAACAGGAACTGAGAAAGACTGTAATGGGACTCAGCCTAAGCCAATGTCAATCACCT
TGAACAGTTCTCAAAGTAATATCTAAATTTTCAATATTTTAAAGTAATATTTGAATCCTA
GAGAATATATCTAAACTGATTTTTCTTTTCTTCTTGTTGTTGTTGTTGTTGTTAGATAAA
TCATCTGATGGAGGAACTAAAAACCGGAAAATCGCGGTAGTCTTCGGTGTAAGCTTGA
Primers used for PCR EQ68 - 8474, show primer details
   
Sequence Name 21-K118578-0022-434-G03C-EQ68
GenBank Accession LN495870 [GenBank]
Sequencing primer gene specific primer, EQ68: 5'-GTCAAGCTTACACCGAAGACTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118578-0022-434-G03C-EQ68
CAACAACAACAACAACAAGAAGAAAAGAAAAATCCAGTTTAGATATATTCTCTAGGATTT
CAAATATTACTTTAAAATATCGAAAATTTAGATATTACTTTGAGAACCGTTCAAGGTGAT
CGACATCGGCTTAGGCTGAGTCCCATTACAGTCTTTCTCAGTTCCTGTTGGACAAATCTG
AGAATTGCCCATAACACTGCATAACAAGTTAAATAAAAACTATATAATATAATTAGGGTA
ACGTATAAAAGTGGATAAATTACAGAGAAAAGAAAAACACAATTACACTGTTACTTTCTC
AGAACAGAGTAAAACAGAGCAGACAAATATTCTTTCTCGTAAATAGACTTACTTGAATGT
TTTGGCAAGTGATCTTGGAACTGGTCCACTCAAGTTATTATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 05-K118577-0022-434-G03C-8409
GenBank Accession LN488563 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118577-0022-434-G03C-8409
GAAAGTAACAGTGTAATTGTGTTTTTTCTTTTTCTCTGTAATTTATCCACTTTTTATACG
TTACCCTAATTATATTATATAGTTTTTATTTAACTTGTTATGCAGTGTTATGGGCAATTC
TCAGATTTGTCCAACAGGAACTGAGAAAGACTGTAATGGGACTCAGCCTAAGCCAATGTC
AATCACCTTGAACAGTTCTCAAAGTAATATCTAAATTTTCAATATTTTAAAGTAATATTT
GAATCCTAGAGAATATATCTAAACTGATTTTTCTTTTCTTCTTGTTGTTGTTGTTGTTGT
TAGATAAATCATCTGATGGAGGAACTAAAAACCGGAAAATCGCGGTAGTCTT
Primers used for PCR KF55 - 3144, show primer details
   
Sequence Name 64-K118684-0022-434-G03C-KF55
GenBank Accession LN511300 [GenBank]
Sequencing primer gene specific primer, KF55: 5'-AATGTTCATCAACAAAATCTTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118684-0022-434-G03C-KF55
GCTTTTCTTTTTTTGTTTCTTCTTTAGTTGTTGCCTTAATAGGAATCAAAAGCTCACTGA
CTGATCCTCATGGAGTTCTAATGAATTGGGATGACACAGCAGTTGATCCATGTAGCTGGA
ACATGATCACTTGTTCTGATGGTTTTGTCATAAGGCTGTAAGCACTTACACACCAACACA
CACACACACACACACATATATGTAATCATGTGTTTACAGTTTTTACTATTGTGTGTATGT
AGAGAAGCTCCAAGCCAAAACTTATCAGGAACTCTTTCATCAAGTATTGGAAATTTAACA
AATCTTCAAACTGTGTAAGTATGTTTTGTAGTAATTATTAAAACATTGTTTCTTCATCTC
TTAATCACTTTAGTTTTCTAATAATTTTGTTTGGAGATACAGGTTATTGCAGAACAATTA
CATAACAGGAAACATCCCTCATGAGATTGGGAAATTGATGAAACTCAAAACACTTGATCT
CTCTACCAATAACTTCACTGGTCAAATCCCATTCACTCTTTCTTACTCCAAAAATCTTCA
GTACTTGTATGATTTTATCCATTTTTTCGTTTTGTTTATTTTATCATATTTTTTATATAA
ATTATTTATGGAGAGATTATTTTTGTGTTGAAAATTGTGGAGCAGGAGGGTTAATAATAA
CAGCCTGACAGGAACAATTCCTAGCTCATTGGCAAACATGACCCAACTCACTTTTTTGTA
CATTCTCTTTCTTTTATTTTCTTCTTTAAATTATATTTTACATATATAATCCCCGGGTAC
CCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAAGATAG
TGGGATTGTGCGTCATCCCTTA
   
Sequence Name 52-K118683-0022-434-G03C-3144
GenBank Accession LN507966 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118683-0022-434-G03C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
NGGGGATTATATATGTAAAATATAATTTAAAGAAGAAAATAAAAGAAAGAGAATGTACAA
AAAAGTGAGTTGGGTCATGTTTGCCAATGAGCTAGGAATTGTTCCTGTCAGGCTGTTATT
ATTAACCCTCCTGCTCCACAATTTTCAACACAAAAATAATCTCTCCATAAATAATTTATA
TAAAAAATATGATAAAATAAACAAAACGAAAAAATGGATAAAATCATACAAGTACTGAAG
ATTTTTGGAGTAAGAAAGAGTGAATGGGATTTGACCAGTGAAGTTATTGGTAGAGAGATC
AAGTGTTTTGAGTTTCATCAATTTCCCAATCTCATGAGGGATGTTTCCTGTTATGTAATT
GTTCTGCAATAACCTGTATCTCCAAACAAAATTATTAGAAAACTAAAGTGATTAAGAGAT
GAAGAAACAATGTTTTAATAATTACTACAAAACATACTTACACAGTTTGAAGATTTGTTA
AATTTCCAATACTTGATGAAAGAGTTCCTGATAAGTTTTGGCTTGGAGCTTCTCTACATA
CACACAATAGTAAAAACTGTAAACACATGATTACATATATGTGTGTGTGTGTGTGTGTGT
TGGTGTGTAAGTGCTTACAGCCTTATGACAAAACCATCAGAACAAGTGATCATGTTCCAG
CTACATGGATCAACTGCTGTGTCATCCCAATTCATTAGAACTCCATGAGGATCAGTCAGT
GAGCTTTTGATTCCTATTAAGGCAACAACTAAAGAAGAAACAAAAAAAGAAAAGCATGTT
TT

Last Updated on 06.12.2018 16:11