Sequences from confirmation amplicon


Confirmation sequences for line 434H01 and hit At1g52670 are shown.
Line ID 434H01
Locus At1g52670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19616176
Primers used for PCR 48fr - 8474, show primer details
   
Sequence Name 24-K111563-0022-434-H01C-48fr
GenBank Accession LN497049 [GenBank]
Sequencing primer gene specific primer, 48fr: 5'-TTCAGATTTGTGGCTTATGAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K111563-0022-434-H01C-48fr
TTCTTCACTACGCTATTGCAGACGTAAAAGATTCTGACGAGACCAAATCAACTGTTGTGA
ATACTCACCTCATGCCCAAGTCCTCCGAGGTAGGTGTTACTTTCCTGTTTAACCTCTCAT
ATTCAAGACCAAGTATGATGCACAACTGTTATATAACCTTAGGGGTTTCAATTTGGGATT
GTTTTGAGAAATCACAAAAGTTAGAAAGTTGCATTTCAGTGCAATAGCACGATACTGTGG
TTCTTTGTTATTATGATGCTAGAGAAGGAAGAATGTGTGACCTCGTTGAGCTAGCTTAGA
CATGAGCTATGTCAAATCTAATCATTATGTTACATTTTCATCTTCTAGGTGGAAGCACTC
ATCAGCGAAATCACCGATTCCTCATCCATTGCAGAGTTTGAACTCAAAGTAAGCCCCACT
TCTGATTTCTGAACTCATAACTATGTTATTTATTGTTGCTCAGATCATTTACTTGTCTCT
GCTAAGTTTTTTCATAAAATCGTGTCTATGATAGAAGTAGTTTTACTATTGACTTAAGTT
TCATCTTAACCATGGTTTATTGTGAAAGTAAACCATGGTTGGTTTAGCTTTTCTGGTTTG
GTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATG
TCCGCA
   
Sequence Name 08-K111556w-0022-434-H01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K111556w-0022-434-H01C-8409
GCATTTCATTGACCAACCAGAAAGCTAAACCAACCATGGTTTACTTTCACAATAAACCAT
GGTTAAGATGAAACTTAAGTCAATAGTAAAACTACTTCTATCATAGACACGATTTTATGA
AAAAACTTAGCAGAGACAAGTAAATGATCTGAGCAACAATAAATAACATAGTTATGAGTT
CAGAAATCAGAAGTGGGGCTTACTTTGAGTTCAAACTCTGCAATGGATGAGGAATCGGTG
ATTTCGCTGATGAGTGCTTCCACCTAGAAGATGAAAATGTAACATAATGATTAGATTTGA
CATAGCTCATGTCTAAGCTAGCTCAACGAGGTCACACATTCTTCCTTCTCTAGCATCATA
ATAACAAAGAACCACAGTATCGTGCTATTGCACTGAAATGCAACTTTCTAACTTTTGTGA
TTTCTCAAAACAATCCCAAATTGAAACCCCTAAGGTTATATAACAGTTGTGCATCATACT
TGGTCTTGAATATGAGAGGTTAAACAGGAAAGTAACACCTACCTCGGAGGACTTGGGCAT
GAGGTGAGTATTCACAACAGTTGATTTGGTCTCGTCAGAATCTTTTACGTCTGCAATAGC
TGTAGTGAAGAATGTTAAAGAAGAAAACAAGCAAACGATACCACTTCATAAGCCAAAAAA
TCT

Last Updated on 06.12.2018 16:11