Sequences from confirmation amplicon


Confirmation sequences for line 435H09 and hit At1g17490 are shown.
Line ID 435H09
Locus At1g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6009017
Primers used for PCR NA55 - 8474, show primer details
   
Sequence Name 39-K101564-0022-435-H09C-8409
GenBank Accession LN503298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K101564-0022-435-H09C-8409
AGCCATTTACATTGAATATATCCTGAAACATCGATTCCTTCTCTTCTTTTCTGCTTCCAA
TCACATTTGTCTTCACCTCTAAAAAAACAACAAAAACCAAAACCCAGAAACGATCAATGA
GATTAAAACAAAAGAGTTGTCTTTTCGCCCAAATTAACCATCTCCTAGATAAACCTAAAT
AACCCAAAAGGTCCCTGATTCGATTACATATAGAAACCCTAAATTCGATAAATCAGGGGG
AATCTCTAGGAGGAATTAGACAAATTAATAAGTGATGAAACTTTATTAAAAAAAAGGGCG
CAAAATTCGTGAAATTGATCAAATCGGAGAATTTGTCTTACCAGAAAGAGAGCTTTGTTT
TGGTTCCGCCATTAATGATCTTCCTTCTTTGATCTTGTTTTTGAGTTCTTCAAATGTAGA
CGAGTTTGTTTAGTTTGGTCTGAGCTTGAAATAGTGTGTACAAATGTGAACCATCCNATG
TAATAATAACAGATTAACGAGTAATTNGAACCG
   
Sequence Name 39-K101577-0022-435-H09C-NA55
GenBank Accession LN503299 [GenBank]
Sequencing primer gene specific primer, NA55: 5'-TCGGTTCGATCTACTCGTTAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K101577-0022-435-H09C-NA55
AGGTTAATTTGTACCCTATTTCAGCTCAGACCAACTAAACAAACTCGTCTACATTTGAAG
AACTCAAAAACAAGATCAAAGAAGGAAGATCATTAATGGCGGAACCAAAACAAAGCTCTC
TTTCTGGTAAGACAAATTCTCCGATTTGATCAATTTCACGAATTTTGCGCCCTTTTTTTT
AATAAAGTTTCATCACTTATTAATTTGTCTAATTCCTCCTAGAGATTCCCCCTGATTTAT
CGAATTTAGGGTTTCTATATGTAATCGAATCAGGGACCTTTTGGGTTATTTAGGTTTATC
TAGGAGATGGTTAATTTGGGCGAAAAGACAACTCTNTTGTTTTAATCTCATTGATCGTTT
CTGGGTTTTGGTTTTTGTTGTTTTTTTAGAGGTGAAGACAAATGTGATTGGAAGCAGAAA
AGAAGAGAAGGAATCGATGTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11