Sequences from confirmation amplicon


Confirmation sequences for line 436C04 and hit At1g25520 are shown.
Line ID 436C04
Locus At1g25520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8962958
Primers used for PCR RK77 - 8474, show primer details
   
Sequence Name 41-K027154-022-436-C04C-8409
GenBank Accession LN503944 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K027154-022-436-C04C-8409
AATTAAACAAAGACATAAGCTTCAAAATCTATGGAGAGAGGTCACTGAAACCAATAGGTC
AAAGCATTCAGAAAAACTTACAATCAGAGCCGATAAACAACCAGCCAACACAAGTCTCCT
AGGATAACGCATAGCCAAAATCTGATATGACAAAAAAAAAAAAGAGTTTAAGCTTGCAAA
ATCAAGATAACACCGGAGGTTTCTTACACCCCTTCAAAGAGCCGGGGGCAAATTCTAAAC
TACCAAAACACTATGAAACTGAAGGGCAATATAAAAGCTACAGACAAAGGATCCAAAGAT
GCCATCTAGATTCTTAATCGGGAAAAGGCAATCCAATAAGTAAGACTTTAGACTTCGATT
CAGGTAAAAGCCATTGACTTCATCGACCTTAAACCCTCAATAGATTACTTGAAGCAACAA
AATCTAAAACTA
   
Sequence Name 45-K027154-022-436-C04C-RK77
GenBank Accession LN505402 [GenBank]
Sequencing primer gene specific primer, RK77: 5'-ATCTTTTAGCTCTTCAGCGAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K027154-022-436-C04C-rk77
GTCTCGTCTATTTGCTCTCTCTCGACGACTGTTAATTAACTCCGGCGATTGATCACTCCG
TCTTCTTTGTAACACAGCCGGAGAAAAATCGAAAGTTTCGTTTTTTTCTTCTCCTTCGTA
GCTAATTCGCCGTCGATTTTATAATTCGGCAGCCATGAGCTCGGTTTTGCAGGTAGAGTA
AAAGATTAATGTTTTCTGGGGAGTAAACTCGTAAGAATCGTTGTGGAGCTAATCTGGGTT
TATGATTGTTGGGGTTTTATAGGGTTTTACGAAGTCACTTGCTATGACCTTTGTATCGGA
GATTGGTGACAAGACGTTTTTCGCTGCAGCAGTAAGTGTTTCGTCTCCTCTTCGCTTTAG
TTTTAGATTTTGTTGCTTCATGTAATCTATTGAGGGTTTAAGGTCGATGAAGTCAATGGC
TTTTACATGAATCGAAGTCTAAAGTCTTACTTATTGGATTGCCTTTTCCTGATTAAGAAT
CTAGATGGCATCTTTGGATCCTTTGTCTGTAGCTTTTATATTGCCATTCAGTTTCATAGT
GTTTTGGTAGTTTAGAATCTGCCACAGGCTCTTTGAAGGTGTGTAAGATACTTCTGTTGT
TATCTTGATTTTGCAAGCTTAAACTCTTTTTTTTTTTTGCCATATCAAATTTTGGCTATG
CGTT

Last Updated on 06.12.2018 16:11