Sequences from confirmation amplicon


Confirmation sequences for line 436G09 and hit At5g10550 are shown.
Line ID 436G09
Locus At5g10550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3334153
Primers used for PCR 12kn - 8474, show primer details
   
Sequence Name 79-K113669-0022-436-G09C-12kn
GenBank Accession LN513854 [GenBank]
Sequencing primer gene specific primer, 12kn: 5'-ATATCCAACTCTATTTCGTCACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K113669-0022-436-G09C-12kn
ATCTGAATCACTGTCCAAGCTTCTCAGGAGGCAATTCTTGTAGATTCACGCCAAGCTTTC
CCTTTTCATCCATTGTCATTTCCCGCTTGTTTGGATCCTTAGCCTTCGGTTTCGGTAACT
TCCCTTTCCTTCCCTTATTCGTTTCACGCACTTCAAGTGATGCTTCCACTTGGTTAACCG
GCTGGGGAGGTGGTGATGGTGGTGGTGGTGGCTGAACCGGTGAAGGAGGAGGAGATGGGT
CACGTGTTATCTCAATTACTGGAGGAGGAGGCAATGTAGGTAGTAGCGGCTTCACTGAAT
CTAGCTTTTTAGCTATAGAAATCTGTTCAGGTCCTTTCCTTGCATTCTCCGCCACATTAT
TCTGATTCCACACTCTTTGATTATCCTCAGGTCCAGGACGAGACGATGATCCCATAACTT
TCACCTCTTGAGCCTCGAATCTCTTCAATGTAGGGTTGAACCAAACGTCAAATTGACTCA
GAAGCTTCTCAGCCATCAAATAAACATCTTGACTTGATGTCCGGCAAAGCGGCAAATCTA
CATGGCTTCATGTCCGGATAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATAAAGATGTCCGCAGCGT
TATA
   
Sequence Name 48-K113666-0022-436-G09C-8409
GenBank Accession LN506556 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K113666-0022-436-G09C-8409
AGATTTGCCGCTTTGCCGGACATCAAGTCAAGATGTTTATTTGATGGCTGAGAAGCTTCT
GAGTCAATTTGACGTTTGGTTCAACCCTACATTGAAGAGATTCGAGGCTCAAGAGGTGAA
AGTTATGGGATCATCGTCTCGTCCTGGACCTGAGGATAATCAAAGAGTGTGGAATCAGAA
TAATGTGGCGGAGAATGCAAGGAAAGGACCTGAACAGATTTCTATAGCTAAAAAGCTAGA
TTCAGTGAAGCCGCTACTACCTACATTGCCTCCTCCTCCAGTAATTGAGATAACACGTGA
CCCATCTCCTCCTCCTTCACCGGTTCAGCCACCACCACCACCATCACCACCTCCCCAGCC
GGTTAACCAAGTGGAAGCATCACTTGAAGTGCGTGAAACGAATAAGGGAAGGAAAGGGAA
GTTACCGAAACCGAAGGCTAAGGATCCAAACAAGCGGGAAATGACAATGGATGAAAAGGG
AAAGCTTGGCGTGAATCTACAAGAATTGCCTCCTGAGAAGCTTGGACAGTTGATTCAGAT
TCTTAGGAAGAGGACAAGAGATTTGCCTCAAGACGGTGACGAAATAGA

Last Updated on 06.12.2018 16:11