Genotyping and primer details


Genotyping and primer details for 436G09 - At5g10550
Line ID 436G09
Locus At5g10550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3334153 of line 436G09 Chr5:3334153
   
Insertion allele amplicon 8474 - 12kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12kn: 5'-ATATCCAACTCTATTTCGTCACCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3333583
   
wt amplicon (over InsPos) 12le - 12kn
wt amplicon start Chr5:3333583
wt amplicon end Chr5:3334716
wt amplicon size 1134 bp
Locus specific primer 12kn: 5'-ATATCCAACTCTATTTCGTCACCG-3'
2nd locus specific primer 12le: 5'-TGGAGACTACGTTTCCTTTGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11