Sequences from confirmation amplicon


Confirmation sequences for line 436H08 and hit At2g24720 are shown.
Line ID 436H08
Locus At2g24720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10524703
Primers used for PCR MM51 - 8474, show primer details
   
Sequence Name 47-K020131-022-436-H08C-MM51
GenBank Accession LN506052 [GenBank]
Sequencing primer gene specific primer, MM51: 5'-CCTCGTCTCACTGATTCATTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K020131-022-436-H08C-MM51
ATATTAATGTTCGGATACCTTATAGATCCGTTATCCCTCTCAATGCCACGGATCAAGATA
TCTCTGTGGAGCTTCTTAAGATGATGAACATGCCCACAAGAGTGTTCATTGTCCACATGT
CCTCTTCTCTAGCCTCAACAGTCTTCATTAAAGCAAAGGAGCTTGGTTTGATGAAACCAG
GATATGTCTGGATCCTTACAAATGGAGTTATGGATGGCTTACGGTCGATAAATGAGACGG
GTATTGAGGCTATGGAGGGGGTATTGGGTATTAAAACTTATATTCCAAAATCCAAAGATC
TAGAAACGTTTAGATCACGTTGGAAGAGGAGATTCCCACAGATGGAGCTGAATGTCTATG
GATTGTGGGCTTATGATGCGACCACTGCACTGGCCATGGCCATTGAAGATGCGGGAATAA
ATAACATGACTTTCAGTAATGTGGATACGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCACGCCC
   
Sequence Name 48-K020131-022-436-H08C-8409
GenBank Accession LN506376 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K020131-022-436-H08C-8409
TAGATTTCCCGTATCCACATTACTGAAAGTCATGTTATTTATTCCCGCATCTTCAATGGC
CATGGCCAGTGCAGTGGTCGCATCATAAGCCCACAATCCATAGACATTCAGCTCCATCTG
TGGGAATCTCCTCTTCCAACGTGATCTAAACGTTTCTAGATCTTTGGATTTTGGAATATA
AGTTTTAATACCCAATACCCCCTCCATAGCCTCAATACCCGTCTCATTTATCGACCGTAA
GCCATCCATAACTCCATTTGTAAGGATCCAGACATATCCTGGTTTCATCAAACCAAGCTC
CTTTGCTTTAATGAAGACTGTTGAGGCTAGAGAAGAGGACATGTGGACAATGAACACTCT
TGTGGGCATGTTCATCATCTTAAGAAGCTCCACAGAGATATCTTGATCCGTGGCATTGAG
AGGGATAACGGATCTATAAGGTATCCGAACATTAATATCTTGTAATGAAT

Last Updated on 06.12.2018 16:11