Sequences from confirmation amplicon


Confirmation sequences for line 438A05 and hit At1g63690 are shown.
Line ID 438A05
Locus At1g63690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23619412
Primers used for PCR EP08 - 8474, show primer details
   
Sequence Name 31-K102174-0022-438-A05C-8409
GenBank Accession LN500103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K102174-0022-438-A05C-8409
ATTTATGCATCAGTTTATTTGGGTATGCTTACTATCACAGACAATATTGTTTGTAGAATT
TAATGGTTTGTATGTTTGTTTGTCTCTGACAGAACTTTACAAAATGGTTTGTGAACCGGA
TGAAACTGATTTAGATATACAGATTCCTGCCGTCATGCTCCCGCAAGATGCTGGTGCATC
CTTGCAAAAGATGCTAGCCAACAGTTCTAAAGGTATGTCCTTTTCTATTTCAATCAGGAT
ATGCTTCCGAAGTTGTCATTTTAAGCTTGTATTGTAAATCATTATATGTATCGGTTAACT
CAACAATCTTCTGCACTTGTCAGTGTCGGCGCAGCTTTACTCCCCAAGACGACCAGCTGT
TGACGTAGCTGAAGTCTTTTTGTGGTTAATGGCAATTGGTACCATTTTATGTGCATCTTA
TTGGTCTGCATGGAGTGCCCGAGAAGCAGCTATTGAACATGATAAGCTACTGAAGGTCAG
ACATTTTCTACTTATTTGGGAATAATAATATTAGGCTAGGAAGATTTAGTTCGATGGGTA
AGGATAACATAAACTGCCAATAAGTAAGTCCTTTGCCCTTCTTGTGCATTTCCAATGATG
CCAAAAGGGACAGAGTAGTTGGTAACTTGGTTAGGCCGAGGAAGAAATCCAAATTGTA
   
Sequence Name 31-K102178-0022-438-A05C-EP08
GenBank Accession LN500104 [GenBank]
Sequencing primer gene specific primer, EP08: 5'-CTACAATTGACTTTTCTTCCTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K102178-0022-438-A05C-EP08
TCTGTCCTTTTGGCATCATTGGAAATGCACAAGAAGGGCAAAGGACTTACTTATTGGCAG
TTTATGTTATCCTTACCCATCGAACTAAATCTTCCTAGCCTAATATTATTATTCCCAAAT
AAGTAGAAAATGTCTGACCTTCAGTAGCTTATCATGTTCAATAGCTGCTTCTCGGGCACT
CCATGCAGACCAATAAGATGCACATAAAATGGTACCAATTGCCATTAACCACAAAAAGAC
TTCAGCTACGTCAACAGCTGGTCGTCTTGGGGAGTAAAGCTGCGCCGACACTGACAAGTG
CAGAAGATTGTTGAGTTAACCGATACATATAATGATTTACAATACAAGCTTAAAATGACA
ACTTCGGAAGCATATCCTGATTGAAATAGAAAAGGACATACCTTTAGAACTGTTGGCTAG
CATCTTTTGCAAGGATGCACCAGCATCTTGCGGGAGCATGACGGCAGGAATCTGTATATC
TAAATCAGTTTCATCCGGTTCACAAACCATTTTGTAAAGTTCTGTCAGAGACAAACAAAC
ATACAAACCATTAAATTCTACAAACAATATTGTCTGTGATAGTAAGCATACCCAAATATA
ACTGATTGCTATAAATTGTAGTGTCCATATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT

Last Updated on 06.12.2018 16:11