Sequences from confirmation amplicon


Confirmation sequences for line 438C06 and hit At4g04790 are shown.
Line ID 438C06
Locus At4g04790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2435010
Primers used for PCR 41vw - 8474, show primer details
   
Sequence Name 19-K118269-0022-438-C06C-41vw
GenBank Accession LN494957 [GenBank]
Sequencing primer gene specific primer, 41vw: 5'-TTCTCGGTCCCAAACAATATTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118269-0022-438-C06C-41vw
TGAACACTCATATTCTTTTTAGGTTTCTTCTTGTTGGGTGCTTGAGAAAATGCAGACCGA
CTTTCTTCGATGATATGTTGTACATCTTTATCATCTATTGGAATTTTTTATATCAATGCC
TTGGCACCTTCTGAATCCCCTGCAGCTAGTAGAACCTGATACATCCTGCCAAACCATAAA
CAAGAACCAATGATATACCGAAAACCTCTTGATCTTTTGGAAACTGATGATCTAGTTATC
AGAATTGACTGCATCCTCTTGAAACTAATCGAACCCGTAAATCGAAACCCACAGGACATT
GTTGAAGAGGAAGACGTTGAGGCAAAGGTGGCTGTAGCAGAGGGTGCTGTAGCAAAGGTG
GCAGAAGTGGTTGTAGCAGAGGTGGCTAAAGCTGAAGTTGTGGCAGACAATCCACGTAAG
AGAAAGACGATTGGTGCTAACTACTATATCCCAGACAAAAGACAACGCTTGTTCTGACTT
TAGGCGGGAGACTCATCTTAGCTCCTTAAGGTTGTTATATAGAAGACTTTAGTTCTGAGA
AAAGAATGATAAATTTGATGTCAAAAAAACAAAAAAGAAAGAAAATTCTTTTTTTTTTAA
TTGGTCAACAAAATATGGACGTCCATATAATATTTCACTTCATAATTACAATATAAAGAA
ATTTTGTACAAAATCACTACTTTAAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGT
   
Sequence Name 04-K118268-0022-438-C06C-oriL
GenBank Accession LN488108 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118268-0022-438-C06C-oriL
GCCATTTACATTTAAAGTAGTGATTTTGTACAAAATTTCTTTATATTGTAATTATGAAGT
GAAATATTATATGGACGTCCATATTCTGTTGACCAATTAAAAAAAAAAGAATTTTCTTTC
TTTTTTGTTTTTTTGACATCAAATTTATCATTCTTTTCTCAGAACTAAAGTCTTCTATAT
AACAACCTTAAGGAGCTAAGATGAGTCTCCCGCCTAAAGTCAGAACAAGCGTTGTCTTTT
GTCTGGGATATAGTAGTTAGCACCAATCGTCTTTCTCTTACGTGGATTGTCTGCCACAAC
TTCAGCTTTAGCCACCTCTGCTACAACCACTTCTGCCACCTTTGCTACAGCACCCTCTGC
TACAGCCACCTTTGCCTCAACGTCTTCCTCTTCAACAATGTCCTGTGGGTTTCGATTTAC
GGGTTCGATTAGTTTCAAGAGGATGCAGTCAATTCTGATAACTAGATCATCAGTTTCCAA
AAGATCAAGAGGTTTTCGGTATATCATTGGTTCTTGTTTATGGTTTGGCAGGATGTATCA
GGTTCTACTAGCTGCAGGGGATTCAAAAGGTGCCAAGGCATTGATATAAAAAATTCCAAT
AGATGATAAAGATGTACAACATATCATCGAAGAAAGTCGGTCTGCATTTTCTCAAGCACC
CAACAAGAAGAAACCTAAAAAGAATATGAGTGTTTCATCAACAAACTACCGTAAAACATC
TATAAGTTAATATTGTT
Primers used for PCR 51wp - 8474, show primer details
   
Sequence Name 25-K118502-0022-438-C06C-51wp
GenBank Accession LN497667 [GenBank]
Sequencing primer gene specific primer, 51wp: 5'-AGCTCGAGTGATCGAGTCTGTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118502-0022-438-C06C-51wp
CCTTCAGTATTAAGCCTCTCAGTGATATGCTGTTTTGGATCAGGTGTTTTGGGCAATCGC
AGAGACAGAGCCATCCAAAGTGCATCTAGGAATGGATTTGCTAAGATTTATGAAAGATGA
GCTCGGGTTTGTTCCTTCAAGAAAATGTCTAGATTTTCTCTTACATGCATGTGTTAATGC
CAAGGACCTGGAGCACGGTCTATTGGTGTGGAAAGAGTACCAATCTGCAGCATTCCCTTG
CAACGTCCTGAGCTTTCTGAGGTAAGCCACTCCTGTTCTCTTTGCTCAAGAACTATAGAT
TTAAGGAACAGAAAGAAAAGATATATCTTGAAATTACATAACAAAAGACCAATTTTGTAC
TATCATTGGCACAAAAAGAGTTACAAGTTTACCACTAGGGTTTTGGTTTACGGGTTCGAT
TAGTTTCAAGTGGATGCAGTTTATTCTGATAATTAGATCATCAGTTTCCAAAAGATCAAG
AGGTTTCCAGTATATCATTGGTTCTGGTTTACGGTTTGGCAGGATGTATCAGGTTCTACT
AGCTGCAGGGGATTCAGAAGGTGCCAAGGCATTGGTGTCAAAAATTCCAAAAGATGATAA
AGATGTACAACATATCATCGAAGAAAGTCAGTCTGCATTTTCTCAAGCACCCAACAAGAA
GAAACCAAAAAAGAAAATGATTGTTTTATCAACAAAGTAGAACAATATGGATCAGCAATA
TACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 09-K118501-0022-438-C06C-8409
GenBank Accession LN490402 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118501-0022-438-C06C-8409
CTTTGTTGATAAAACATCATTTTCTTTTTTGGTTTCTTCTTGTTGGGTGCTTGAGAAAAT
GCAGACTGACTTTCTTCGATGATATGTTGTACATCTTTATCATCTTTTGGAATTTTTGAC
ACCAATGCCTTGGCACCTTCTGAATCCCCTGCAGCTAGTAGAACCTGATACATCCTGCCA
AACCGTAAACCAGAACCAATGATATACTGGAAACCTCTTGATCTTTTGGAAACTGATGAT
CTAATTATCAGAATAAACTGCATCCACTTGAAACTAATCGAACCCGTAAACCAAAACCCT
AGTGGTAAACTTGTAACTCTTTTTGTGCCAATGATAGTACAAAATTGGTCTTTTGTTATG
TAATTTCAAGATATATCTTTTCTTTCTGTTCCTTAAATCTATAGTTCTTGAGCAAAGAGA
ACAGGAGTGGCTTACCTCAGAAAGCTCAGGACGTTGCAAGGGAATGCTGCAGATTGGTAC
TCTTTCCACACCAATAGACCGTGCTCCAGGTCCTTGGCATTAACACATGCATGTAAGAGA
AAATCTAGACATTTTCTTGAAGGAACAAACCCGAGCTCATCTTTCATAAATCTTAGCAAA
TCCATTCCTAGATGCACTTTGGATGGCTCTGTCTCTGCGATTGCCCAAAACACCTGATCC
AAAACAGCATATCACTGAGAGGCTTAAATTACTGAAGGCTAATTCAGTAAAAGAGAACAT
AGCTACTAGTTA

Last Updated on 06.12.2018 16:11