Sequences from confirmation amplicon


Confirmation sequences for line 438E07 and hit At1g16400 are shown.
Line ID 438E07
Locus At1g16400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5606771
Primers used for PCR CC24 - 8474, show primer details
   
Sequence Name 47-K106187-0022-438-E07C-CC24
GenBank Accession LN506204 [GenBank]
Sequencing primer gene specific primer, CC24: 5'-CGGAGATTCTTAGAAAAGCTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K106187-0022-438-E07C-CC24
GCTTGTGCAGAATCGACATACGAAATCTAAACTACTTAAAAGCTTGTTGCAGAGAAACAT
TCAGGATTCACCCAAGCGCTCATTATGTCCCACCTCATGTTGCCCGTCAAGATACCACCC
TTGGGGGTTATTTTATTCCCAAAGGTAAAACATATAGGGAATACATATGTTTTTTATTTA
CATAAGATTTAACGTTTATAGCTAGTAAGTTTATGCATGAGCTTCGTTAACAAAATAAAA
CACATGGTTTTGGTTTGTGGCTACATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 31-K106188-0022-438-E07C-8409
GenBank Accession LN500172 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K106188-0022-438-E07C-8409
TCGGACATGAGCCATTTACAATTGAATTGTAGCCACAAACCAAAACCATGTGTTTTATTT
TGTTAACGAAGCTCATGCATAAACTTACTAGCTATAAACGTTAAATCTTATGTAAATAAA
AAACATATGTATTCCCTATATGTTTTACCTTTGGGAATAAAATAACCCCCAAGGGTGGTA
TCTTGACGGGCAACATGAGGTGGGACATAATGAGCGCTTGGGTGAATCCTGAATGTTTCT
CTGCAACAAGCTTTTAAGTAGTTTAGATTTCGTATGTCTGATTCTTGCACAAGCCTGTCT
TTTCCAACTACTTCATCCAACTCCTTCAGAGCTTTTCTAGNAATCTCC

Last Updated on 06.12.2018 16:11