Sequences from confirmation amplicon


Confirmation sequences for line 438H03 and hit At4g33270 are shown.
Line ID 438H03
Locus At4g33270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16044602
Primers used for PCR JL18 - 8474, show primer details
   
Sequence Name 52-K032418-022-438-H03C-8409
GenBank Accession LN507713 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K032418-022-438-H03C-8409
CCATTTACATTGAATATTTCCAAAACATTCCAGAACCTCAGAGTCTCATCTCCTGCTGCT
GAAGCTACGGTACAACCATCTGGACTCTGTAAGAAATACAACACAAACAACACAAGTTTT
AGGACTAAAGACCTCAAACATCTCCAACTACTTTCAACAGTTAAAAATTGATTTAGTTGT
TTTCCTTTACCTGGGCCATATATAGAACTCTTGATGTATGACCAGTGAGCTCAGCCATTT
TCACCATGGATGGATACTTCCAAAGTGTGAGCTGATTCTGTGTAAACCCGTGTGAGCTAA
GCAACTCTCTTTCATTCTTGCTCCATAACAACGAACAAACTTGGGAACCAGTGTCTACTG
AATTCAAGCAAGCCCCAGTGTGAGTATTCCAGAACTTGATCGTCCTGTCTCCTCCACCAC
CACCAGTTGCAAGCAAATTCGCTTGGAAAGGGCACCACGCAAGAGCCTTCACAGCAGATG
TATGTTCCTCAAGCCTGTGCAGCCATTGTGTGGTTGAGTTTGAGGAAGCGACAGAACGAT
CCCAGATGTGTACCACATTGTCGTTGCCACCACTTGCTAGTTGTTGTCCAGATCCTGACC
ACTTGAGCCCACAAACTTCTTGAGTGTGACCTCTGTAAGTTTCCACAATGGGTGATCTGA
TCCTCACATCATTGTTGATGATCAGTCCATCCATTCCTCCAGTAGTAAGGATGTGATTGT
TCCATGCCAGTGATCCTACTCGTGACTGGTGACCACCCTTCAATGTTCTCAGCTGCAAGT
ATATGATAAAACACATGAGACTAAGAACACCGGATATTATATCTCAACATAAAAGAGAGA
GAGTAGTAATTACTTGACGGTTGGATGCAGAATCCCACAGCTGGACTTCAGAGTGTG
   
Sequence Name 52-K032418-022-438-H03C-JL18
GenBank Accession LN507714 [GenBank]
Sequencing primer gene specific primer, JL18: 5'-CAGTTGGACTCAACAACTCTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K032418-022-438-H03C-JL18
CAGTAATTACTACTCTCTCTCTTTTATGTTGAGATATAATATCCGGTGTTCTTAGTCTCA
TGTGTTTTATCATATACTTGCAGCTGAGAACATTGAAGGGTGGTCACCAGTCACGAGTAG
GATCACTGGCATGGAACAATCACATCCTTACTACTGGAGGAATGGATGGACTGATCATCA
ACAATGATGTGAGGATCAGATCACCCATTGTGGAAACTTACAGAGGTCACACTCAAGAAG
TTTGTGGGCTCAAGTGGTCAGGATCTGGACAACAACTAGCAAGTGGTGGCAACGACAATG
TGGTACACATCTGGGATCGTTCTGTCGCTTCCTCAAACTCAACCACACAATGGCTGCACA
GGCTTGAGGAACATACATCTGCTGTGAAGGCTCTTGCGTGGTGCCCTTTCCAAGCGAATT
TGCTTGCAACTGGTGGTGGTGGAGGAGACAGGACGATCAAGTTCTGGAATACTCACACTG
GGGCTTGCTTGAATTCAGTAGACACTGGTTCCCAAGTTTGTTCGTTGTTATGGAGCAAGA
ATGAAAGAGAGTTGCTTAGCTCACACGGGTTTACACAGAATCAGCTCACACTTTGGAAGT
ATCCATCCATGGTGAAAATGGCTGAGCTCACTGGTCATACATCAAGAGTTCTATATATGG
CCCAGGTAAAGGAAAACAACTAAATCAATTTTTAACTGTTGAAAGTAGTTGGAGATGTTT
GAGGTCTTTAGTCCTAAAACTTGTGTTGTTTGTGTTGTATTTCTTACAGAGTCCAGATGG
TTGTACCGTAGCTTCAGCAGCAGGAGATGAGACTCTGAGGTTCTGGAATGTTTTTGGAAA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAGAAAGAGTAATACCAATTTTTTTTCATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAGTACATTTTGATAAAC
Primers used for PCR JL17 - 8474, show primer details
   
Sequence Name 51-K032523-022-438-H03C-8409
GenBank Accession LN507403 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032523-022-438-H03C-8409
AAAGCTGCTCCAAAGCAGTTTCCGAGCCATTTTCTCACGTGAATCGTATTCGTTGAAGCT
GTGAAGACTGACGGACAAGGAAACCCATGATACAGATGCAAAATATTCATTTTTTTTGCA
TCTGATTCATTCTTCATGAATTGTAGTGACAAGAAAAGTGCATATTGTACAATTATATAT
ATGTAAATGATATATATTGTTGAGTCAAACTTATTTGCTTGCATTTGAATAGAAAGAAAG
ATCTTGATTATATATGTCAGATGATACAAGAAGCAGGAGCATTAAGTACTGTAAACTGTA
AGATGATAATTGTAACTACATCAAAATAGACATTGAAACCACACTAACCTAATCACATTA
GTGTGTAGAAACATGTGTTCGATTAGGGATTTTGGAAGATTTTATTTTTTCCGCTAGCTT
ATGTAATTTCAGGGATTTTAGAAGATTTTGGTTTTATGTTTTTAAACCACTTGTTATGTT
TTCAGGTTAATGAATTGATTAACAAATGCTTTTTTTGGATATAAATTTTTTATATGATTA
AAATAATACTAAAACAATATTATAGGTGAAAAATAC
   
Sequence Name 51-K032523-022-438-H03C-JL17
GenBank Accession LN507404 [GenBank]
Sequencing primer gene specific primer, JL17: 5'-TGTGTGATCGGTATTTTTCACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K032523-022-438-H03C-JL17
TCATATAAAATTTATATCCAAAAAAGCATTTGTTAATCAATTCATTAACCTGAAAACATA
ACAAGTGGGTTTAAAAACATAAAACCAAAATCTTCTAAAATCCCTGAAATTACATAAGCT
AGCGGAAAAAATAAAATCTTCCAAAATCCCTAATCGAACACATGTTTCTACACACTAATG
TGATTAGGTTAGTGTGGTTTCAATGTCTATTTTGATGTAGTTACAATTATCATCTTACAG
TTTACAGTACTTAATGCTCCTGCTTCTTGTATCATCTGACATATATAATCAAGATCTTTC
TTTCTATTCAAATGCAAGCAAATAAGTTTGACTCAACAATATATATCATTTACATATATA
TAATTGTACAATATGCACTTTTCTTGTCACTACAATTCATGAAGAATGAATCAGATGCAA
AAAAAATGAATATTTTGCATCTGTATCATGGGTTTCCTTGTCCGTCAGTCTTCACAGCTT
CAACGAATACGATTCACGTGAGAAAATGGCTCGGAAACTGCTTTTGGAGCAGCTTTTTTG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTT
Primers used for PCR 29lf - 8474, show primer details
   
Sequence Name 71-K113920-0022-438-H03C-8409
GenBank Accession LN512596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K113920-0022-438-H03C-8409
GCCAAAAGCTGCTCCAAAAGCAGTTTCCGAGCCATTTTCTCACGTGAATCGTATTCGTTG
AAGCTGTGAAGACTGACGGACAAGGAAACCCATGATACAGATGCAAAATATTCATTTTTT
TTGCATCTGATTCATTCTTCATGAATTGTAGTGACAAGAAAAGTGCATATTGTACAATTA
TATATATGTAAATGATATATATTGTTGAGTCAAACTTATTTGCTTGCATTTGAATAGAAA
GAAAGATCTTGATTATATATGTCAGATGATACAAGAAGCAGGAGCATTAAGTACTGTAAA
CTGTAAGATGATAATTGTAACTACATCAAAATAGACATTGAAACCACACTAACCTAATCA
CATTAGTGTGTAGAAACATGTGTTCGATTAGGGATTTTGGAAGATTTTATTTTTTCCGCT
AGCTTATGTAATTTCAGGGATTTTAGAAGATTTTGGTTTTATGTTTTTAAACCACTTGTT
ATGTTTTCAGGTTAATGAATTGATTAACAAATGCTTTTTTTGGATATAAATTTTTTATAT
GATTAAAATAATACTAAAACAATATTATAGGTGAAAAATACCGATCACACAATAACCATT
ATTTAACCATAAAGCAAATATTCAATCACACAATAAACCTA
   
Sequence Name 61-K113919-0022-438-H03C-29lf
GenBank Accession LN510576 [GenBank]
Sequencing primer gene specific primer, 29lf: 5'-GTTAGGTTATTGTGTGATTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113919-0022-438-H03C-29lf
TCGGTATTTTTCACCTATATATTGTTTTAGTATTATTTTAATCATATAAAAAATTTATAT
CCAAAAAAAGCATTTGTTAATCAATTCATTAACCTGAAAACATAACAAGTGGTTTAAAAA
CATAAAACCAAAATCTTCTAAAATCCCTGAAATTACATAAGCTAGCGGAAAAAATAAAAT
CTTCCAAAATCCCTAATCGAACACATGTTTCTACACACTAATGTGATTAGGTTAGTGTGG
TTTCAATGTCTATTTTGATGTAGTTACAATTATCATCTTACAGTTTACAGTACTTAATGC
TCCTGCTTCTTGTATCATCTGACATATATAATCAAGATCTTTCTTTCTATTCAAATGCAA
GCAAATAAGTTTGACTCAACAATATATATCATTTACATATATATAATTGTACAATATGCA
CTTTTCTTGTCACTACAATTCATGAAGAATGAATCAGATGCAAAAAAAATGAATATTTTG
CATCTGTATCATGGGTTTCCTTGTCCGTCAGTCTTCACAGCTTCAACGAATACGATTCAC
GTGAGAAAATGGCTCGGAAACTGCTTTTGGAGCAGCTTTTTTGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11