Sequences from confirmation amplicon


Confirmation sequences for line 439B11 and hit At1g37150 are shown.
Line ID 439B11
Locus At1g37150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:14176545
Primers used for PCR JH31 - 8474, show primer details
   
Sequence Name 54-K101270-0022-439-B11C-8409
GenBank Accession LN508417 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101270-0022-439-B11C-8409
GAGCCTTTCGTTTACTTAGAATGGAAGATGGTCGAGTAGTGCCTTTGATACAGTATGTGG
TGTCTCTTGCTGTTACTGAGGCAGTGAAAGATGTTTGTGACAAGAAGGTATGTTCTTGAA
AATAACCTTTTTGTTGTGTGTTTTGATCCCTTGTATCAGAAATTTGAAGTTCATTTGTTT
TCAGGGTTTGTCGTATAATGATGTTAAAATAAAGTGGCCAAATGATCTATACTTGAATGG
CCTTAAGATTGGAGGCATTTTGTGTACCTCTACATATAGATCAAGGAAGTTCCTTGTCAG
TGTTGGTAATAAGCATTACTCTTTAGAAGTTTGTAATAAGTATCTTTTAGAAGTAGCACT
GCTCTTAGTCTTATTGAAGCTGGTGTTCCTTC
   
Sequence Name 54-K101276-0022-439-B11C-JH31
GenBank Accession LN508418 [GenBank]
Sequencing primer gene specific primer, JH31: 5'-AAGAAGGAACACCAGCTTCAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K101276-0022-439-B11C-JH31
TTATTACAAACTTCTAAAGAGTAATGCTTATTACCAACACTGACAAGGAACTTCCTTGAT
CTATATGTAGAGGTACACAAAATGCCTCCAATCTTAAGGCCATTCAAGTATAGATCATTT
GGCCACTTTATTTTAACATCATTATACGACAAACCCTGAAAACAAATGAACTTCAAATTT
CTGATACAAGGGATCAAAACACACAACAAAAAGGTTATTTTCAAGAACATACCTTCTTGT
CACAAACATCTTTCACTGCCTCAGTAACAGCAAGAGACACCACATACTGTATCAAAGGCA
CTACTCGACCATCTTCCATTTCTAATGTAAACGAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA

Last Updated on 06.12.2018 16:11