Sequences from confirmation amplicon


Confirmation sequences for line 439E10 and hit At2g01820 are shown.
Line ID 439E10
Locus At2g01820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:360108
Primers used for PCR NA66 - 8474, show primer details
   
Sequence Name 05-K101499-0022-439-E10C-8409
GenBank Accession LN488290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101499-0022-439-E10C-8409
CATTTACATTGAATATATCCTGAAACTATCTCAGAATGGTTTAGAAGGAGAGTTACCGAT
GAGTTTTGCTGGTACTTCGATTCAGTCTCTGTTCTTGAATGGTCAGAAGCTTAATGGATC
GATCTCGGTATTGGGGAACATGACTTCGCTCGTTGAGGTTTCTCTTCAAGGAAATCAGTT
CTCAGGTCCAATTCCTGATCTCTCTGGTTTGGTATCTCTACGTGTGTTTAATGTTAGAGA
GAATCAGCTCACTGGTGTTGTTCCACAGTCTTTGGTTAGTTTGAGTTCGCTTACTACTGT
GAATTTAACTAATAATTATCTTCAAGGACCGACTCCNTTGTTTGGCAAATCCGTTGGTGT
TGATATAGTCAACAACATGAATAGCTNTTGTACTAATGTAGCTGGTGAGGCTTGTGATCC
TCGTGTGGATACTTTGGTATCTGTAGCTGAATCATTTGGATATCCNGTGAAGCTTGCGGA
AAGTTGGAAAGGAAACAATCCTTGTGTTAACTGGGTTGGGATTACCTGTTCA
   
Sequence Name 05-K101503-0022-439-E10C-NA66
GenBank Accession LN488291 [GenBank]
Sequencing primer gene specific primer, NA66: 5'-GAACAAGTAATCCCAACCCAGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K101503-0022-439-E10C-NA66
CTTTCACTTTCCGCAGCTTCACTGGATATCCAATGATTCAGCTACAGATACCAAAGTATC
CACACGAGGATCACAAGCCTCACCAGCTACATTAGTACAAAAGCTATTCATGTTGTTGAC
TATATCAACACCAACGGATTTGCCAAACAACGGAGTCGGTCCTTGAAGATAATTATTAGT
TAAATTCACAGTAGTAAGCGAACTCAAACTAACCAAAGACTGTGGAACAACACCAGTGAG
CTGATTCTCTCTAACATTAAACACACGTAGAGATACCAAACCAGAGAGATCAGGAATTGG
ACCTGAGAACTGATTTCCTTGAAGAGAAACCTCAACGAGCGAAGTCATGTTCCCCAATAC
CGAGATCGATCCATTAAGCTTCTGACCATTCAAGAACAGAGACTGAATCGAAGTACCAGC
AAAACTCATCGGTNACTCTCCTTCTAAACCATTCTGAGATAGTTTCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTATTACCAATTTTTTTTCATTCAAAATGTAGATACCGCACGT

Last Updated on 06.12.2018 16:11