Sequences from confirmation amplicon


Confirmation sequences for line 440E01 and hit At5g25020 are shown.
Line ID 440E01
Locus At5g25020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8619445
Primers used for PCR 53rn - 8474, show primer details
   
Sequence Name 31-K116083-0022-440-E01C-53rn
GenBank Accession LN500301 [GenBank]
Sequencing primer gene specific primer, 53rn: 5'-CTGGGAAGGCTATGAAGTGTAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K116083-0022-440-E01C-53rn
ATGTTGATATAAGTAGCTCGGCGATTATGACGGCGCTTATTAGGTTTATGTTGGGGTATG
TTACGTATTTAATGGTTGATATTGGTTTTGTTGTGGAAGCGCAAACGGAGGAGGAGTTGC
CGGAGAGATTGATTGGTGGTGCTAGGATTTGTCATATGGAGTTATCGTCGGCGTTTGTGA
TTTATGATGATGTTGATGGGAAGAAGAGTTCGCAGCAACGGCGGAGAATGATGGGTGCGG
CGGAAGGAAATCTACATGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTAATTAT
   
Sequence Name 15-K116082-0022-440-E01C-8409
GenBank Accession LN493125 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116082-0022-440-E01C-8409
CATTTACATTGAATATATCCTGCATGTAGATTTCCTTCCGCCGCACCCATCATTCTCCGC
CGTTGCTGCGAACTCTTCTTCCCATCAACATCATCATAAATCACAAACGCCGACGATAAC
TCCATATGACAAATCCTAGCACCACCAATCAATCTCTCCGGCAACTCCTCCTCCGTTTGC
GCTTCCACAACAAAACCAATATCAACCATTAAATACGTAACATACCCCAACATAAACCTA
ATAAGCGCCGTCATAATCGCCGAGCTACTTATATCAACATCAACCTCAAAATAATTATCT
CCTCTATGATAACTACACTTCATAGCCTTCCCAGA

Last Updated on 06.12.2018 16:11