Sequences from confirmation amplicon


Confirmation sequences for line 440E08 and hit At2g25160 are shown.
Line ID 440E08
Locus At2g25160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10711217
Primers used for PCR NU18 - 8474, show primer details
   
Sequence Name 67-K104197-0022-440-E08C-NU18
GenBank Accession LN511806 [GenBank]
Sequencing primer gene specific primer, NU18: 5'-TATGAACTCTTGCTTCATGTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K104197-0022-440-E08C-NU18
TCTTTTCTCCTCTACGTATAAGGCTCTAAATGCCAAGTCAGCCTCACGTGCTCTACTAAC
CATGTGCATATCAACCCCGGATGTCGAGAAAAGCTCGGATACTGCAATCTTCCGTATCTC
TCTCCAGTAAAGACCGTAAGGACTAAACGTCAAGAACGAGTCGTTGTAGCCTAGAAGCTT
AGACGCAGTAAGTTCTGGTCGTTCTAGGAGCTTGTCGTGGACCGTGTAAATCTCTTTGGC
CACTTCTTTGCTGTTTATGATCATGACTTTGATAGAGCCAAGCTTTGCCATGAACACCGG
TCCATACACGTCAGCCATTGCCCCAAACGTAACGTGAGTCGGGTTTACGGTGTCAAATAA
ATGTAAATGACCGAGCAATGGCCATGCTCCGGGGACCATAGGTGCTGTACTCTTCTTGTT
CTTGGGACGAAGCCGAGACTTAATTAAGATTAAAACAGGGAAGATGAAGAGAGCAGAGAG
AAGAAAAAGCATGATTAGATCCATGTATGGATAATTTTCTGCAAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 77-K104198-0022-440-E08C-8409
GenBank Accession LN513543 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K104198-0022-440-E08C-8409
CCATTTTGCGAAATTATCCATACATGGATCTAATCATGCTTTTTCTTCTCTCTGCTCTCT
TCATCTTCCCTGTTTTAATCTTAATTAAGTCTCGGCTTCGTCCCAAGAACAAGAAGAGTA
CAGCACCTATGGTCCCCGGAGCATGGCCATTGCTCGGTCATTTACATTTATTTGACACCG
TAAACCCGACTCACGTTACGTTTGGGGCAATGGCTGACGTGTATGGACCGGTGTTCATGG
CAAAGCTTGGCTCTATCAAAGTCATGATCATAAACAGCAAAGAAGTGGCCAAAGAGATTT
ACACGGTCCACGACAAGCTCCTAGAACGACCAGAACTTACTGCGTCTAAGCTTCTAGGCT
ACAACGACTCGTTCTTGACGTTTAGTCCTTACGGTCTTTACTGGAGAGAGATACGGAAGA
TTGCAGTATCCGAGCTTTTCTCGACATCCGGGGTTGATATGCACATGGTTAGTAGAGCAC
GTGAGGCTGACTTGGCATTTAGAGCCTTATACGTTAGATGGGAGAAAAGAGGTAAGCCTA
AAGAAGGAGTCTTGGTGGACATGAAGCAGAAGTTCAT

Last Updated on 06.12.2018 16:11