Sequences from confirmation amplicon


Confirmation sequences for line 440H12 and hit At3g46590 are shown.
Line ID 440H12
Locus At3g46590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17156164
Primers used for PCR 58ra - 8474, show primer details
   
Sequence Name 33-K115914-0022-440-H12C-58ra
GenBank Accession LN501132 [GenBank]
Sequencing primer gene specific primer, 58ra: 5'-CACTTTCTCTTCTTCATGTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115914-0022-440-H12C-58ra
CTTTCGAGGAACGCAGACCATCCGTACCTATGTTGATTCTTCAAGGTTAGTTAGTTTTTT
AACTAACTCTTAACCTTAATATATACAGTTTGGTATTGAGTCTTTGCCTTTGTAGGATAA
GTGGAAGACGCTGGTCCACACGGCAAGGATATCTCCACAACAGCGGAGAGGAGAGCCGGT
GCCACAGGAACTTCTGGACCGTGTTTTGAAGGCACACGCTTACTGGTCACAACACCTAAT
GCATCAACTGCAGACTGAGCCACCATCCACTCAGGTAGAGGCTCTCTAATCTGAACAGGT
TCTTGGTCTAAAGAACCTTTGTGTATTATTATGATGATCCATATTGTAAGATTCTGACAT
AGTTGAAGATCTGGTCTCAGTTCTTCTTTGTTTTTTTTTTTCTTTCTTCCAAAGGAAAAG
TTTTATAATTGAAAATAAGGTTGGGTTTTTTCTAACAATGTAAAGGATTTGTTGTAAATC
TTTGGGGACATTTTTATTCTGCCTATATAATTTAGGGCTCGGCCTATCATGTCCGGAAAA
TCTACTTGGATCACCAATGATTATGATGGTCAATATGGAAAAAAAAAAAAAGTATTTCCC
ATTTTTTTTTCATTTCAAAAATGTA
   
Sequence Name 07-K115913-0022-440-H12C-8409
GenBank Accession LN489420 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115913-0022-440-H12C-8409
TATAGACAGAATAAAAATGTCACCAAAGATTACAACAAATCCTTTACATTGTTAGAAAAA
ACCCAACCTTATTTCCAATTATAAAACTTTACATTTGAAAGAAAGAAAAAAAAAACCAAA
GAAGAACTGAGACCAGATCTTCAACTATGTCAAAATCTTACAATATGGATCATCATAATA
ATACACAAAGGTTCTTTAAACCAAAAACCTGTTCAAATTAAAGAGCCTCTACCTGAGTGG
ATGGGGGCTCATTCTGCAGTTGATGCATTAGGTGTTGTGACCAGTAAGCGTGTGCCTTCA
AAACACGGTCCAAAAGTTCCTGTGGCACCGGCTCTCCTCTCCGCTGTTGTGGAAATATCC
TTGCCGTGGGGACCAGCGTCTTCCACTTATCCTACAAAGGCAAAAACTCAATACCAAACT
GTATATATTAAGGTTAAAAGTTAGTTAAAAAACTAACTAACCTTGAAATCAACATAGGTA
CAATGGTCTGCGTCCTCAAAAGCACAAACCTTCACATCTCTCCACCTGTAGTTTTCAAAC
ACATTTTGTCACCAACATGAAAAAAAA

Last Updated on 06.12.2018 16:11