Sequences from confirmation amplicon


Confirmation sequences for line 441E03 and hit At5g61240 are shown.
Line ID 441E03
Locus At5g61240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24631212
Primers used for PCR 42by - 8474, show primer details
   
Sequence Name 77-K108523-0022-441-E03C-42by
GenBank Accession LN513562 [GenBank]
Sequencing primer gene specific primer, 42by: 5'-AAACTCAGGATCCAATTCTCTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K108523-0022-441-E03C-42by
GAGTCTGTTATTTGTGCCATCATAACATGTCAAGTTTCCAATACTTCACCTAAACATTTA
CTCTGTTGTTATCACTAGAAACCTGAGGTGGAATAAACTACAAGATGTGATCCCTCCCGA
GATTGGGGAGCTGAAGAGACTAACCCATTTGTGAGTACCTTAATGTTTCTTTTTACCTTC
TGATGATCGTCCATTTTTATCCACATGAAATCATGATATTTTTTCTTCTTTTCTTTTTAT
GTTTAAGGTACTTGAGCTTCAATAGCTTCAAGGGAGAAATTCCCAAGGAACTAGCTGCTC
TTCCCGAGCTTCGGTATCTATACCTTCAAGAAAACCGTCTCATTGGTAGAATTCCTGCCG
AACTGGGGACTCTGCAAAACCTTCGCCACCTGTAAACACCAAATGCCGTTTTCTACATAT
AGTTTGGGTACCCTCCAATGATTTATTTACTGAGATCATGTTTGGTTTGTGTCCCATTAT
TTTTCAGAGATGTTGGTAACAACCATTTGGTGGGAACTATACGGGAGCTCATCCGTTTTG
ATGGAAGTTTCCCAGCTCTTCGCAACTTGTAAGCATCCCTTCAGAAATTTCCATTTGCTT
CTCTCTTCCGTTTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCTATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 64-K108522-0022-441-E03C-8409
GenBank Accession LN511218 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K108522-0022-441-E03C-8409
CATTTACAATTGAATATATCAAACGGAAGAGAGAAGCAAATGGAAATTTCTGAAGGGATG
CTTACAAGTTGCGAAGAGCTGGGAAACTTCCATCAAAACGGATGAGCTCCCGTATAGTTC
CCACCAAATGGTTGTTACCAACATCTCTGAAAAATAATGGGACACAAACCAAACATGATC
TCAGTAAATAAATCATTGGAGGGTACCCAAACTATATGTAGAAAACGGCATTTGGTGTTT
ACAGGTGGCGAAGGTTTTGCAGAGTCCCCAGTTCGGCAGGAATTCTACCAATGAGACGGT
TTTCTTGAAGGTATAGATACCGAAGCTCGGGAAGAGCAGCTAGTTCCTTGGGAATTTCTC
CCTTGAAGCTATTGAAGCTCAAGTACCTTAAACATAAAAAGAAAAGAAGAAAAAATATCA
TGATTTCATGTGGATAAAAATGGACGATCATCAGAAGGTAAAAAGAAACATTAAGGTACT
CACAAATGGGTTAGTCTCTTCAGCTCCCCAATCTCGGGAGGGATCACATCTTGTAGTTTA
TTCCACCTCAGGTTTCTAGTGATAACAACAGAGTAAATGTTTAGGTGAAGTATTTGGAAA
CTTGACATGTTATGATGGCACAAATAACAGACTTCTGTCACGTAAGTGCTAAGTTCACTC
TGAAGAGAATTGGATCCTGAGT

Last Updated on 06.12.2018 16:11