Sequences from confirmation amplicon


Confirmation sequences for line 444F07 and hit At1g48510 are shown.
Line ID 444F07
Locus At1g48510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17936335
Primers used for PCR au36 - 3144, show primer details
   
Sequence Name 05-K106829-0022-444-F07C-3144
GenBank Accession LN488373 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106829-0022-444-F07C-3144
CCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTTATTGCACTAGTAACT
GGTTGTTTTTGTTTGTTTGTTTATGGTAGTGTGCCTGATTGTTTCTATATGCAGGCACTG
GAGTTCCCTGACTTGCTTTATTAAGGCGTCTTCGATTTTAATGCGAAGATTAACAAAATC
TGATCCTATTGGTGTAGAGCCAATATTGATTCCAATTTCCATTCTCGTTTTTATATGTAC
CAAGATATACAGTCTTCGTAACTTATTTTGTAAAATAGACACTATTGGTGTTGGGTGTGT
CACTAAACTCGACACAGGATAAGTCAAA
   
Sequence Name 10-K106830-0022-444-F07C-au36
GenBank Accession LN490675 [GenBank]
Sequencing primer gene specific primer, au36: 5'-ATTTGACTTTTCCTGTGTCGAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K106830-0022-444-F07C-au36
TTTACAAATAAGTTACGAAGACTGTATATCTTGGTACATATAAAAACGAGAATGGAAATT
GGAATCAATATTGGCTCTACACCAATAGGATCAGATTTTGTTAATCTTCGCATTAAAATC
GAAGACGCCTTAATAAAGCAAGTCAGGGAACTCCAGTGCCTGCATATAGAAACAATCAGG
CACACTACCATAAACAAACAAACAAAAACAACCAGTTACTAGTGCAATAACTGATAGTTT
AAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAAT
GAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTT
ACGTCAGTGGAGATATCACATCAATCCA
Primers used for PCR CC31 - 8474, show primer details
   
Sequence Name 18-K106287-0022-444-F07C-CC31
GenBank Accession LN494317 [GenBank]
Sequencing primer gene specific primer, CC31: 5'-ATTGTTGAGGTTGGTCTTCATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106287-0022-444-F07C-CC31
ATCATTCATTGCATGATTTGATATCATAGTTAAGTGTTATTGGAATATTTTTTAGCAGGA
CCAAGTCTCTAGGGCTATGCATGTGGAAGTGGTGGGTGTAGTTAGGAAAAGTGAAACTCC
AGGCATATATACTCTAGTAAATTATCCAAGTTCGCTTGCGTGGTTCTACCTTGACGTTCC
TAAACTAGCTCTAGCCATGGGGTTTGGTGAGGACACAATGTATATAGAGAGTACGTACAC
AGATATGGATGAAAGTAGAACTTATCCAGTTCCAAGAGACGTTGAGAACTTGACTCGTAG
TAAGGATATACCACTGGACTATCACTTGTACACAGTTTTTACAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 18-K106286-0022-444-F07C-8409
GenBank Accession LN494316 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106286-0022-444-F07C-8409
ATTTACATTGTAAAACTGTGTACAGTGATAGTCCAGTGGTATATCCTTACTACGAGTCAA
GTTCTCAACGTCTCTTGGAACTGGATAAGTTCTACTTTCATCCATATCTGTGTACGTACT
CTCTATATACATTGTGTCCTCACCAAACCCCATGGCTAGAGCTAGTTTAGGAACGTCAAG
GTAGAACCACGCAAGCGAACTTGGATAATTTACTAGAGTATATATGCCTGGAGTTTCACT
TTTCCTAACTACACCCACCACTTCCACATGCATAGCCCTAGAGACTTGGTCCTGCTAAAA
AATATTCCAATAACACTTAACTATGATATCAAATCATGCAATGAATGATTGAGTACTCAT
TAAGTTTATGAAATGAAGACCAACNTCAACAA

Last Updated on 06.12.2018 16:11