Genotyping and primer details


Genotyping and primer details for 444F07 - At1g48510
Line ID 444F07
Locus At1g48510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17936335 of line 444F07 Chr1:17936335
   
Insertion allele amplicon 8474 - CC31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC31: 5'-ATTGTTGAGGTTGGTCTTCATTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17935953
   
Insertion allele amplicon 3144 - au36
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer au36: 5'-ATTTGACTTTTCCTGTGTCGAGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17936649
   
wt amplicon (over InsPos) au36 - CC31
wt amplicon start Chr1:17935953
wt amplicon end Chr1:17936649
wt amplicon size 697 bp
Locus specific primer CC31: 5'-ATTGTTGAGGTTGGTCTTCATTTC-3'
2nd locus specific primer au36: 5'-ATTTGACTTTTCCTGTGTCGAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11