Sequences from confirmation amplicon


Confirmation sequences for line 445B11 and hit At5g46760 are shown.
Line ID 445B11
Locus At5g46760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18975841
Primers used for PCR JL10 - 8474, show primer details
   
Sequence Name 54-K032418-022-445-B11C-8409
GenBank Accession LN508377 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032418-022-445-B11C-8409
GCCATTTACATTGATTCATTTACTCATTGTACACGTATCATCACATCCCAACCTATGATC
TTAACATCAATCTCCATTTCTATAGAACTCGCCGTAGAATCTTGATTCGAACTTTTCCGT
TCTTTTGCCCTTGACCCGCAACCTTTCCCATTATTCCCTTCCTTACTCATCCCATCTAGC
TTCTTCTGAATCTCCTCTTTATCAGACTCCGCTTGCTGCAGCTTCGACTTAAGCTCATTG
ATATACGAAATCGCGTCTCCGAGAAGCGAAGCTTTATCCATCTTCGAAACGTTAGGAACA
ACAGCTCTCAAAGAGTAGAATCTCTGGTTTAACTTCTCTCTTCTCTGCCTCTCTGCTTCA
ACATGATTCAACGGCTCTTCTCTCCCATTCGCCGGTTTCCTCCCCCGTTTCCTCGGCTTC
TTCTCCGGTGGCTCAACAACAATCGCTTCCTTAACAACAGATGCTTCAAGATCAGAATGA
TCCGAATCATTCGCAGCTGATCTAACCACAGTACTAAACGAAAGCATCCCTTCATCATTA
TTACTCCCTTTAGATACCGAAGTTCTCTTCTTACTACTCTCATTACCACAGAAACTCAAA
GTCTCATTAGATTT
   
Sequence Name 54-K032418-022-445-B11C-JL10
GenBank Accession LN508378 [GenBank]
Sequencing primer gene specific primer, JL10: 5'-AAGGTGGGTTGTTGAAATCTAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K032418-022-445-B11C-JL10
AAGAGACTTCGGTATCTAAAGGGAGTAATAATGATGAAGGGATGCTTTCGTTTAGTACTG
TGGTTAGATCAGCTGCGAATGATTCGGATCATTCTGATCTTGAAGCATCTGTTGTTAAGG
AAGCGATTGTTGTTGAGCCACCGGAGAAGAAGCCGAGGAAACGGGGGAGGAAACCGGCGA
ATGGGAGAGAAGAGCCGTTGAATCATGTTGAAGCAGAGAGGCAGAGAAGAGAGAAGTTAA
ACCAGAGATTCTACTCTTTGAGAGCTGTTGTTCCTAACGTTTCGAAGATGGATAAAGCTT
CGCTTCTCGGAGACGCGATTTCGTATATCAATGAGCTTAAGTCGAAGCTGCAGCAAGCGG
AGTCTGATAAAGAGGAGATTCAGAAGAAGCTAGATGGGATGAGTAAGGAAGGGAATAATG
GGAAAGGTTGCGGGTCAAGGGCAAAAGAACGGAAAAGTTCGAATCAAGATTCTACGGCGA
GTTCTATAGAAATGGAGATTGATGTTAAGATCATAGGTTGGGATGTGATGATACGTGTAC
AATGAGTAAATGAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATNTCGACGTGTCTACT
Primers used for PCR FP72 - 8474, show primer details
   
Sequence Name 57-K035339-022-445-B11C-8409
GenBank Accession LN509340 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K035339-022-445-B11C-8409
TGAGCCATTTAAATTGAATTTACAATTGATTGGATGTAGGTTCATGGAAGCACTTAAGGA
ATTGGATTTGGAAGTGAATCATGCGAGTTTATCCGTTGTGAATGATTTGATGATTCAACA
AGCTACGGTGAAGATGGGGAGCCAATTTTTCAATCATGACCAGCTCAAAGTTGCTTTGAT
GACGAAAGTCGGAGAAAACTATTGAACAAAGTTGGCATATCAAAGAGGGGAAACTAGTGT
TTTCATCGATGAAATGGGATCGAATTTAGCGGGTAATGTATGGTCTTTTGTAAAGTAGTA
AGTACTCATTTTGCATCACTCGCTTCATGTTGTTAAATGTTGTTGTTCATTAGGAGAACT
TGCTTAGTTCTTAGCTTAGTGTTATCATAAGCCTTAAGTGAGAAGGAAGTTGGAGACTAG
AAGACCAGAGAGAGTTAGCGAAATAATAAAATCTTTGGTTGTACTTGTATGTATATATAT
GTATGGAGTAAGATTAAGTGAGATCCTAAATTCTATTGAAGTTTTTTTATTCTATGATTG
AATTTTTTTGTTAGTTTGTAACTTTGTAGTGTACATAAAGGTCCAAATTTATGAACTGTA
GTGTACCAAAAAGCTGAGGAAAATAAGTG
   
Sequence Name 57-K035339-022-445-B11C-FP72
GenBank Accession LN509341 [GenBank]
Sequencing primer gene specific primer, FP72: 5'-CCACTTATTTTCCTCAGCTTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K035339-022-445-B11C-FP72
TTTGGACCTTTATGTACACTACAAAGTTACAAACTAACAAAAAAATTCAATCATAGAATA
AAAAAACTTCAATAGAATTTAGGATCTCACTTAATCTTACTCCATACATATATATACATA
CAAGTACAACCAAAGATTTTATTATTTCGCTAACTCTCTCTGGTCTTCTAGTCTCCAACT
TCCTTCTCACTTAAGGCTTATGATAACACTAAGCTAAGAACTAAGCAAGTTCTCCTAATG
AACAACAACATTTAACAACATGAAGCGAGTGATGCAAAATGAGTACTTACTACTTTACAA
AAGACCATACATTACCCGCTAAATTCGATCCCATTTCATCGATGAAAACACTAGTTTCCC
CTCTTTGATATGCCAACTTTGTTCAATAGTTTTCTCCGACTTTCGTCATCAAAGCAACTT
TGAGCTGGTCATGATTGAAAAATTGGCTCCCCATCTTCACCGTAGCTTGTTGAATCATCA
AATCATTCACAACGGATAAACTCGCATGATTCACTTCCAAATCCAATTCCTTAAGTGCTT
CCATGAACCTACATCCAATCAATTGTAAATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAGATCTTTATTTCGACGTGTCTACATTCCTCCCCAA

Last Updated on 06.12.2018 16:11