Sequences from confirmation amplicon


Confirmation sequences for line 446E01 and hit At4g00160 are shown.
Line ID 446E01
Locus At4g00160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:63465
Primers used for PCR 12qr - 8474, show primer details
   
Sequence Name 54-K115714-0022-446-E01C-12qr
GenBank Accession LN508583 [GenBank]
Sequencing primer gene specific primer, 12qr: 5'-AGAGAATCTTCAAGTACATCGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K115714-0022-446-E01C-12qr
TCGGATTGGTCATTTAGGGGTTTACTTCTTAGAGATGGTTAACATTTTGGTATTAATAAG
TAAACCGATCGGATTCGGTTCGAATTTAAACAAAACCGAGATATTGAATGAGAAGCCCTA
GTCTTATCACATTCTTCTACTCGTTGAAATGCGAATAGTCATTAGGGATTCAGTTTTGTT
GAAATGCGATTTTAGCACTGGAAAAACATAAGCCTGAAGGATTTCACCGACAAAGATTGT
AAATTGTAAATGGATCAAACACTGATAGTTTAAACCTACATGTTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 14-K115713-0022-446-E01C-8409
GenBank Accession LN492646 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115713-0022-446-E01C-8409
GTTTAACTATCAGTGTTTGATCCATTTACAATTTACAATCTTTGTCGGTGAAATCCTTCA
GGCTTATGTTTTTCCAGTGCTAAAATCGCATTTCAACAAAACTGAATCCCTAATGACTAT
TCGCATTTCAACGAGTAGAAGAATGTGATAAGACTAGGGCTTCTCATTCAATATCTCGGT
TTTGTTTAAATTCGAACCGAATCCGATCGGTTTACTTATTAATACCAAAATGTTAACCAT
CTCTAAGAAGTAAACCCCTAAATGACCTAATCCGATGAATGACCTAATCCGATGATCGAC
CTAGTTCCCGATGTACTTAAAGATTCTCT

Last Updated on 06.12.2018 16:11